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FluoroFinder News & Updates  

From flow cytometry research and experimental design trends to FluoroFinder tool updates and industry applications, we explore it all in our blog.  

Newsletter: Antigen Density Explained

Newsletter: Antigen Density Explained

This month we explain why antigen density is important to flow cytometry studies, describe how it is measured, and list some useful references for known antigen expression densities... 1. Why Antigen Density Matters 2. Methods for Determining Antigen Density 3....

Newsletter: 7 Steps of a Successful Flow Cytometry Experiment

Newsletter: 7 Steps of a Successful Flow Cytometry Experiment

Getting started in flow cytometry may seem overwhelming, so we’ve broken the flow cytometry experiment down into seven basic steps. 1. Select your Cytometer 2. Design your panel 3. Optimizing your staining protocol                                                 4....

Newsletter: The History of Flow Cytometry

The field of flow cytometry research has evolved significantly over the past 70 years. Here we take a look back at some of the most important achievements on the path to bigger, faster and better flow cytometry experiments. Microscopes (Early 1700s) Antonie van...

Newsletter: Tandem Dyes

Newsletter: Tandem Dyes

The commercial availability of antibody-linked fluorescent dyes has expanded rapidly over the past decade. Where researchers were once limited to a handful of common dyes (FITC, PE, APC, PerCP, etc.), they can now choose from an extensive list of fluorescent dye lines...

Newsletter: Compensation

Newsletter: Compensation

Proper compensation is critical for accurate interpretation of your flow cytometry data. Therefore, we have compiled this “comprehensive” list of compensation tips to help improve your analysis. Sections: What is Compensation & Why Do I Need it? Best Practices for...

Newsletter: The Fight Against Irreproducibility

Newsletter: The Fight Against Irreproducibility

The “reproducibility crisis”, or the inability for researchers to replicate results or reproduce findings remains a growing concern for a wide range of scientific disciplines. This month, the National Association of Scholars published an excellent report examining the...

Newsletter: Tips for Getting Published

Newsletter: Tips for Getting Published

Posted on: Apr 3, 2018 We know you put a lot of time and effort into your flow cytometry experiments. Follow these 7 tips to ensure that your high-quality flow data is more likely to be published! Select the right cytometer Prepare your samples correctly Design a...

Newsletter: OMIPs Simplify Panel Design

Newsletter: OMIPs Simplify Panel Design

What is a dump channel? Simply put, a dump (also called an exclusion) channel is used to group and exclude everything that is not of interest for your study. This typically involves using one or more antibodies to stain antigens that are known not to be expressed by...

Newsletter: Dump Channels

Newsletter: Dump Channels

What is a dump channel? Simply put, a dump (also called an exclusion) channel is used to group and exclude everything that is not of interest for your study. This typically involves using one or more antibodies to stain antigens that are known not to be expressed by...

Newsletter: Viability Dye Selection Guide

Newsletter: Viability Dye Selection Guide

Cell viability dyes are critical controls for proper flow cytometry analysis. Dead cells can skew data by causing cell aggregation, contributing to cellular autofluorescence or nonspecifically binding detection antibodies. This is especially problematic when measuring...

Newsletter: Art of Gating Flow Cytometry Data

Newsletter: Art of Gating Flow Cytometry Data

Flow cytometry gating can often seem like a daunting task. While there is no single solution, experienced cytometrists can recommend several tips beyond “praying for good data”. Here we define gating, explore gating methodologies, and provide some useful tips for...

Newsletter: Colors

Newsletter: Colors

This month we explore the ever-expanding selection of commercially available fluorescent antibodies and how researchers are getting better data by designing more colorful panels. Sections: Research Trend Toward More Colors Suppliers Expanding Fluorescent Catalogs...

Newsletter: Best Practices

Newsletter: Best Practices

FluoroFinder has integrated cytometer configurations for over 500 cores! (You can check for yours here). With so many researchers now using FluoroFinder to design their flow cytometry experiments, we wanted to share some of the excellent feedback we have received....

Newsletter: Get More from Your Core

Newsletter: Get More from Your Core

If you are working with a shared flow core facility, then you probably know how important it is to get the most out of your limited cytometer time. Aside from costing your lab time and grant money, shared cytometers are a valuable resource so booking additional time...

Newsletter: Reproducibility Crisis

Newsletter: Reproducibility Crisis

You may have read about a growing “reproducibility crisis”, where scientists are increasingly unable to replicate the results of their colleagues’ scientific experiments. To combat this trend, research teams are emphasizing collaboration throughout the experiment...

Cytometers

Cytometers

Analyzers & Cell Sorters Selecting the optimal cytometer for your experiment can be as important as the panel you design. We have compiled this useful list of popular cytometers for your reference. However, you should also consult your flow core manager for their...

Newsletter: Common Mistakes

Newsletter: Common Mistakes

We have compiled tips to void the 6 most common pitfalls of cytometry experiment design and analysis. Learn how to avoid failed experiments, save time and frustration and get better data! Sections: Reagent/Fluorochrome Selection Controls Sample Prep Spillover...

Newsletter: Flow Dictionary

Newsletter: Flow Dictionary

Note: The flow dictionary is not alphabetical, but rather grouped by the logical order of a cell as it flows through a cytometer. If you are looking for a particular term, it may be easiest to use the ctrl+f search function. Sections: Fluidics Optics & Detection...

Newsletter: Immunobeads

Newsletter: Immunobeads

Understanding Flow Cytometry Beads Immunobeads, or antibody-coated nanoparticles, are useful for a variety of flow cytometry-related applications. Here we outline the most commonly used bead types and explain how they can be used for cytometer calibration,...

Newsletter: Pre-Sort Checklist

Are you planning to run a cell sort on a precious sample? Concerned about recovery? Be sure to review our pre-sort checklist of 7 things experienced cytometrists recommend to improve cell recovery: Cell Suspension Reduce Cell “Stickiness” Monitor Cell Conditions...

Newsletter: OMIP's

Designing a multicolor fluorescent panel can take months to develop and optimize. Fortunately, Optimized Multicolor Immunofluorescence Panels (OMIPs) have greatly reduced this process. Now, researchers can recreate any published OMIP on FluoroFinder and easily edit...

Newsletter: Tracking Fluorescent Proteins

Are you designing multicolor panels around your fluorescent proteins? Would seeing your fluorescent proteins and reagents in a multi-vendor spectra viewer help you better anticipate issues? FluoroFinder enables you to add fluorescent proteins to your multicolor panel...

Newsletter: Validation Data

Newsletter: Validation Data

Could a new product improve your experiment? Has that new antibody been vetted by reliable testing? Researchers have seen tremendous growth in the number of commercially available marker and dye combinations designed for flow cytometry. As new products come online,...

Newsletter: Viability Dyes

Drug treatments, genetic manipulations and simply handling cells can all have an effect on the health of your samples.  It is important to take these effects into consideration when analyzing your flow cytometry experiment. Dead cells can skew your results by...

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