OMIPS

OMIP Panels Based on Cell Population Being Profiled 

Optimized Multicolor Immunofluorescence Panels (OMIPs) are peer-reviewed panels designed for fluorescent assays.  Researchers can access OMIPS pre-loaded in the FluoroFinder panel builder and design a similar panel or OMIP. Start by selecting “Build an OMIP Panel” on your OMIP of choice below. Next, select a compatible instrument and proceed through the steps to design your own version of the OMIP.

CD4 OR CD8 T CELLS

OMIP-001: Quality and phenotype of Ag-responsive human T-cells

OMIP-002: Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes

OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells

OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells

OMIP-009: Characterization of antigen-specific human T-cells

OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice

OMIP-013: differentiation of human T-cells

OMIP-014: validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining

OMIP-015: human regulatory and activated T-cells without intracellular staining

OMIP-016: Characterization of antigen-responsive macaque and human T-cells

OMIP-017: human CD4(+) helper T-cell subsets including follicular helper cells

OMIP-018: chemokine receptor expression on human T helper cells

OMIP-020: Phenotypic characterization of human γδ T-cells by multicolor flow cytometry

OMIP-021: Simultaneous quantification of human conventional and innate-like T-cell subsets

OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory

OMIP-023: 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes

OMIP-024:  Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples

OMIP-025: evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining

OMIP-030: Characterization of human T cell subsets via surface markers

OMIP-031: Immunologic checkpoint expression on murine effector and memory T-cell subsets

OMIP-033: A comprehensive single step staining protocol for human T- and B-cell subsets

OMIP-034: Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies

OMIP-036: Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets

OMIP-042: 21-color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood

OMIP-050: A 28-color/30-parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules

OMIP-052: An 18-Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques

OMIP-056: Evaluation of Human Conventional T Cells, Donor-Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining

OMIP-058: 30-Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells

OMIP-060: 30-Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T Cells

OMIP‐062: A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples

OMIP-063: 28-Color Flow Cytometry Panel for Broad Human Immunophenotyping

OMIP-067: 28-Color Flow Cytometry Panel to Evaluate Human T-Cell Phenotype and Function

OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

OMIP 071: A 31-Parameter Flow Cytometry Panel for In-Depth Immunophenotyping of Human T-Cell Subsets Using Surface Markers

OMIP 072: A 15-color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemia

OMIP 073: Analysis of human thymocyte development with a 14-color flow cytometry panel

OMIP 075: A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primates

OMIP 076: High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsets

OMIP 078: A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells

B CELLS

OMIP‐003: Phenotypic analysis of human memory B cells

OMIP‐026: Phenotypic analysis of B and plasma cells in rhesus macaques

OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland

OMIP‐033: A comprehensive single step staining protocol for human T‐ and B‐cell subsets

OMIP‐043: Identification of human antibody secreting cell subsets

OMIP‐047: High‐Dimensional phenotypic characterization of B cells

OMIP‐051:  28‐color flow cytometry panel to characterize B cells and myeloid cells

OMIP‐068: High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic Infection

OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

OMIP-074: Phenotypic analysis of IgG and IgA subclasses on human B cells

OMIP 077: Definition of all principal human leukocyte populations using a broadly applicable 14-color panel

NK CELLS

OMIP‐007: Phenotypic analysis of human natural killer cells

OMIP-024:  Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples

OMIP‐027: Functional analysis of human natural killer cells

OMIP‐028: Activation panel for Rhesus macaque NK cell subsets

OMIPS-029: Human NK-cell phenotypization

OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland

OMIP‐035: Functional analysis of natural killer cell subsets in macaques

OMIP‐037: 16‐color panel to measure inhibitory receptor signatures from multiple human immune cell subsets

OMIP‐039: Detection and analysis of human adaptive NKG2C+ natural killer cells

OMIP‐064: A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells

OMIP‐070: NKp46‐Based 27‐Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues

DENDRITIC CELLS (DCS)

OMIP‐038: Innate immune assessment with a 14 color flow cytometry panel

OMIP‐044: 28‐color immunophenotyping of the human dendritic cell compartment

OMIP‐051: 28‐color flow cytometry panel to characterize B cells and myeloid cells

OMIP‐061: 20‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting Cells

OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

REGULATORY T CELL (TREG)

OMIP‐004: In‐depth characterization of human T regulatory cells

OMIP‐006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry

OMIP‐015: Human regulatory and activated T‐cells without intracellular staining

OMIP-048: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry

OMIP‐053: Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg Subsets

INNATE LYMPHOID CELLS (ILC)

OMIP‐055: Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood

OMIP‐066: Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood

OMIP‐062: A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples

OMIP‐064: A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells

OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

OTHER HEMATOPOIETIC/IMMUNE CELLS

OMIP‐010: A new 10‐color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples

OMIP‐019: Quantification of human γδT‐cells, iNKT‐cells, and hematopoietic precursors

OMIP-046: Characterization of invariant T cell subset activation in humans

OMIP‐049: Analysis of Human Myelopoiesis and Myeloid Neoplasms

OMIP‐059: Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining Panel

 OMIP-065: Dog Immunophenotyping and T-Cell Activity Evaluation with a 14-Color Panel

TISSUE DERIVED CELLS

OMIP‐040: Optimized gating of human prostate cellular subpopulations

OMIP‐041: Optimized multicolor immunofluorescence panel rat microglial staining protocol

OMIP‐045: Characterizing human head and neck tumors and cancer cell lines with mass cytometry

OMIP-054:  Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry

OMIP-057: Mouse γδ T-Cell Development Characterized by a 14 Color Flow Cytometry Panel

 References and Collaborators:

1. Wei Wang, MD. The Columbia Center for Translational Immunology (CCTI) at Columbia University Irving Medical Center.” Cytometry Part A

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