Flow Cytometry Training

On-Demand Training

This streamlined, brand-neutral educational program is delivered as subtitled video-based content and simulation.  Beginner and advanced users can take advantage of the extensive course content to gain a full understanding of fundamental concepts and advanced specialty topics. This program is also a great resource to prepare for the Specialist in Cytometry, SCYM (ASCP) exam and generates 8 points toward the certification renewal.

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Program OUTLINE

Check the program outline for an overview of the topics covered in each eCourse.

1. Principles and Instrumentation
  1. What is Flow Cytometry?
  2. Common Applications
  3. Fluorescence
    1. Light Spectrum
    2. Absorption & Emission
    3. Fluorochrome Classes
    4. Fluorochrome Properties
  4. Fluidics 
    1. Flow
    2. Pressure & Flow Rate
    3. Acoustic-Assisted Focusing
  5. Optics
    1. Excitation
    2. Light Collection
    3. Photo Detection
  6. Electronics
    1. Amplification & Conversion
    2. Voltage Pulse
    3. Time Delay
    4. Digitization and Binning
  7. FCS File
  8. Section Summary
  9. Quiz (15 questions)
2. Reagents and Panel Design
  1. Sample Requirements
  2. Cellular Markers & Dyes
  3. Fluorescence Detection
  4. Fluorochrome Selection
  5. Antigen Classification
  6. Antibody Titration
  7. Dead Cell Staining
  8. Exclusion/Dump Channel
  9. Spectral Overlap
  10. Compensation Controls
  11. Compensation
  12. Spectral Overlap Reduction
  13. Spillover Spreading
  14. Spillover Spreading Matrix
  15. Autofluorescence
  16. Nonspecific Binding
  17. Fluorescence Minus One Controls
  18. Biological Controls
  19. Replicates
  20. Section Summary
  21. Quiz (15 questions)

3. Acquisition and Measurements
  1. Plots & Gates
  2. Scaling
  3. Acquisition Speed
  4. Event Cycling
  5. Scatter Setup
  6. Threshold & Trigger
  7. Aggregate Discrimination
  8. Dead Cell Exclusion
  9. Fluorescent Parameter Setup
  10. Compensation
  11. Data Recording
  12. Data Exporting
  13. Assay Setup Validation
  14. Instrument Cleaning
  15. Section Summary
  16. Quiz (15 questions)
4. Analysis (with interactive Tools)
  1. Matrix Inspection
  2. Data Display
    1. Histograms & Plots
    2. Gates, Regions, Quadrants
    3. Gating
  3. Biexponential Scaling
  4. Data Cleaning
    1. Time Gating
    2. Debris Exclusion
    3. Aggregates & Dead Cells
    4. Autofluorescence
    5. Fluorochrome Aggregates
    6. Impact
  5. Gate Validation
    1. FMO Controls
    2. Backgating/Color Gating
  6. Cell Cycle
    1. One-color DNA Analysis
    2. Multi-color DNA Analysis
  7. Section Summary
  8. Interactive Tools
    1. Matrices
    2. Plotting
    3. Gating
    4. Scaling
    5. Cleaning Impact
    6. Controls
    7. DNA Analysis
  9. Quiz (15 questions)
5. Statistics
  1. Number of Events to Record
  2. Descriptive Statistics
    1. Distribution
    2. Central Tendency
    3. Dispersion
    4. Data Normalization
    5. Fold Change
  3. Inferential Statistics
    1. Statistical Test
    2. Statistical Power
    3. Hypotheses
    4. Significance Level
    5. Statistical Errors
    6. Probability Value
    7. Student’s t-test
      1. T-distribution and Freedom
      2. Formula
      3. Real-Life Example pt1
      4. Real-Life Example pt2
  4. Section Summary
  5. Quiz (15 questions)
6. Cell Sorting
  1. What is Cell Sorting? Process Overview
  2. Nozzle Size & Sheath Pressure –  Droplet Generation
  3. Droplet Breakoff
  4. Stream Charging & Deflection
  5. Collection
  6. Instrument Optimization –  Drop Delay
  7. Acquisition & Gating
  8. Conflicts
  9. Sort Decisions
  10. Sorting 
  11. Post-Sort – Purity, Recovery & Yield
  12. Process Stressors – Sample Quality
  13. Temperature & Pressure – Sample Media & Sheath
  14. Collection Media – Instrument Variables
  15. Sort Performance – Sort Quality
  16. Collection Issues  – Contamination
  17. Single-Cell Deposition
  18. Biosafety – Summary
  19. Quiz (20 questions)
7. Full-Spectrum Flow Cytometry
  1. What is Full-Spectrum Flow Cytometry?
  2. Full-Spectrum vs. Conventional
  3. Spectral Emission Signature
  4. Signature Comparison
  5. Signature Alterations
  6. Reference Controls 
  7. Spectral Reference Library
  8. Instrument Settings 
  9. Acquisition
  10. Spectral Profile & Unmixing
  11. Autofluorescence Management
  12. Unmixing Errors
  13. Analysis
  14. Panel Optimization
    1. Introduction
    2. Design
    3. Testing
    4. Evaluation & Remediation
  15. Summary
  16. Quiz (20 questions)

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