FluoroFinder’s Fluorescent Dye Database compiles detailed information on the optical properties and spectral profiles of more than a thousand fluorochromes. Enter your instrument laser and filter combinations on the interactive spectra viewer and find the best fluorochrome for your application.
FluoroFinder’s fluorophore collection comprises organic molecules and fluorescent proteins from all suppliers to provide the most complete and up-to-date collection of product information available for any fluorescence-based applications. The aim is to provide the life-science community with a tool that integrates fluorochrome data with product selection in one platform, including:
- Excitation and emission profiles, commonly used laser lines and filters, photostability, and brightness are collected in one resource
- Interactive Spectra Viewer to display instrument configurations alongside spectral properties of fluorochromes
- Comprehensive list of available antibodies labeled with fluorescent dyes, conjugation kits, and labeling services
Fluorescence detection is used in many different applications. The selection of fluorescent dyes is based on the type of application and the instrument used for detection. The optical characteristics of fluorescent dyes need to be compatible with the experimental design and instrument settings.
Flourescent Dye and Experiment Design
Fluorophores are molecules that emit fluorescence upon excitation with a light source with a shorter wavelength. They can be categorized as organic dyes, fluorescent proteins, and quantum dots and are defined by their optical and chemical properties:
- Excitation and emission peaks
- Stoke’s shift: the difference (in nanometers) between the peak excitation and the peak emission wavelengths.
- Molar extinction coefficient: a measure of how strongly a chemical species or substance absorbs light at a particular wavelength
- Quantum yield: defined as the ratio of the number of photons emitted to the number of photons absorbed
- Brightness: depends on the molar extinction coefficient and the quantum yield
- PH sensitivity
Fluorophores listed on FluoroFinder’s database are used in all fluorescence-based applications:
- Flow Cytometry
- Fluorescence Microscopy and Imaging
- Calcium Transient measurements
- Cytosolic pH quantification
- Apoptosis and cell viability and proliferation
- Signal transduction
- Cell signaling