Flow Cytometry

The development of increasingly powerful instruments has expanded the number of parameters that can be simultaneously detected and analyzed. At the same time, an exploding number of available fluorophores and labeled antibodies has contributed to the complexity of the technology. Beginners and seasoned researchers alike can benefit from using FluoroFinder’s intuitive experiment design platform. Tools for panel design and spectral visualization are integrated with a comprehensive collection of fluorophores and labeled antibodies.

FluoroFinder has collected the resources needed to get started with flow cytometry experiment design in one platform:

Panel design

  • Pre-loaded instrument configurations.
  • Antigen density and fluorophore brightness,
  • Integrated excitation and emission spectra for over 1,000 fluorophores.
  • Technical specification and product reviews for over 3 million antibodies.
  • Save and share experimental design.
  • Spectral flow cytometry panel design functionality,

Spectra Viewer

  • Instrument-specific laser and filter configurations.
  • Interactive interface to compare the spectral properties of fluorochromes to facilitate the selection of dyes compatible with instrument laser and filter configurations, minimizing spectral overlay.

Cytometers and cell-sorters

  • Listing all technical specifications and comparing the general performance of the different types of commercially available instruments.

Analysis software

Provides an overview of all the available tools and the applications they are suitable for.

Dye database

Over 1,000 fluorophores from all suppliers including product technical specifications and spectral properties.

OMIPS

Optimized Multicolor Immunofluorescence Panels.  

 

Applications of FLOW CYTOMETRY

Flow cytometry is a widely used technology that simultaneously measures and analyzes multiple physical characteristics of single cells as they flow in a fluid stream through a beam of light. The properties measured include cells’ relative size and granularity or internal complexity, and the relative fluorescence intensity associated with cellular marker expression. Flow cytometry relies on the use of fluorescently-conjugated antibodies to bind to and recognize specific cellular markers and to fluorescent dyes that bind directly to nucleic acids. The technology has applications in numerous fields, making it an essential analysis tool in diagnostic, clinical, and research labs. Flow cytometry is used for:

  • Immunophenotyping
  • Cell sorting 
  • Cell cycle analysis
  • Apoptosis
  • Cell proliferation assay
  • Intracellular calcium flux
  • Transfection efficiency
  • Virology
  • Cancer diagnosis
flow cytometry

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