This collection of webinars speaks to experimental design across industries that utilize tools that FluoroFinder offers on our platform. We regularly update these webinars as an invaluable resource for our community of users.
What’s the Deal with Your Compensation and Unmixing?
Speaker: Andreas (Jack) Panopoulos, PhD, Slingshot Biosciences, Inc.
In this webinar, we discuss the powerful rules governing proper compensation controls and employment of the spillover spreading matrix to reduce errors and avoid loss of resolution, respectively.
A Serum-Free, Chemically-Defined Workflow for T Cell Therapy
Speaker: Zayda Piedra, PhD, Product Development Scientist at BioLegend
In this webinar, we will go over current challenges in the cell therapy industry and what we can do better, BioLegend’s emerging solutions for T cell therapy – closing the gaps, one at a time, and the importance of bioprocessing reagents manufactured with consistent, high-quality well-defined components.
Highly Multiplexed Imaging of Diverse Tissues Using IBEX
Speaker: Andrea Radtke, Ph.D. and Dr. Ziv Yaniv, Ph.D.
In this webinar, we will go over methods to study normal and malignant lymph nodes and topics related to multiplexed tissue imaging, namely: optimal tissue processing, working with fixed frozen and FFPE specimens, overcoming autofluorescence, identifying appropriate reagents, validating antibodies, and creating organ mapping antibody panels (OMAPs).
MA900 Cell Sorter: Increasing Application Capabilities in Multi-User Laboratories
Speaker: Anthony Carcio, Field Application Scientist, Sony Biotechnology
This webinar will provide insights to researchers who are new to sorting or who operate shared flow facilities on how the Sony MA900 can support varied user needs and guided workflows for sorting. Learn how an automated and streamlined sort setup can increase the capabilities within a multi-user lab, review how the MA900 enables seamless setup and exchange of different chips without instrument downtime between users, and understand how the MA900 can be maintained in multi-user labs for successful, high-throughput, and gentle sorting of various cells.
Evaluation of Large Antibody Panels in Single-Cell Genomic Immunophenotyping of Fresh and Frozen Human Leukocytes
Speaker: Dr. Jasmine Ying-Jiun Chen
Dr. Chen will share Abiosciences’ effort in evaluating BioLegend’s TotalSeq™-C Human Universal Cocktail using the 10x Genomics single-cell platform on fresh and frozen PBMCs. This includes bioinformatics pipelines to compare the quality control metrics, isotype matched control performance, and cell subtype analyses. Advanced visualization and analysis tools were developed to interrogate the data in a flow cytometry paradigm, including complex gating strategies to identify rare cell subtypes. The pilot study is being expanded to larger scale clinical settings and may help characterize important cell populations that are associated with disease status, pharmacodynamics, and therapeutic response.
Cell Painting – A Practical Approach
Speaker: Joe Trask, North America HCS Product Line Leader, Cellular Imaging and Analysis, PerkinElmer, and Alexander Schreiner Team Leader, Biological Applications Team, PerkinElmer, Hamburg, Germany
Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology. Specific cellular compartments and organelles are simultaneously stained with six fluorescent probes, followed by imaging and analysis to extract hundreds to thousands of features to phenotypically profile the cells and gain a deeper understanding of the effects of chemical, drug, or genetic perturbagens.
High-Dimensional High-Throughput Rare Event Immunophenotyping on the ID7000™ Spectral Cell Analyzer
Speaker: Jeff Clapper, Senior Field Applications Scientist Sony Biotechnology Inc.
The ID7000™ spectral cell analyzer is ideal for high-dimensional flow cytometry. More lasers and many more detectors mean more fluorochromes can be resolved in combination.
Software Workflows and Tools that Enable High Parameter Flow Cytometry using the ID7000™ Spectral Cell Analyzer
Speaker: Vendula Sinkorova, Senior Field Application Scientist, Sony Biotechnology Inc.
In this webinar from Sony, we will review the workflows to highlight the ease of operating the ID7000™ spectral cell analyzer to obtain high-parameter, high-quality data.
Measuring T Cell Exhaustion: A Multiplexed Approach Utilizing Advanced Flow Cytometry
Speaker: Mark Carter, Manager of Assay Development. Product Development Cell Analytics, Sartorius
Understanding immune system dynamics and their role in health and disease, as well as developing novel therapies, relies on the use of powerful analytical screening tools to probe cellular functions. Characterizing immune cell function such as the induction and persistence of T cell exhaustion is important for furthering therapeutic interventions.
AccuCheck ERF Reference Particles: Easy to Use Flow Cytometry Intensity Reference with Accurate NIST-Assigned Values
Adam W. York, Ph.D. Sr. Staff Scientist in Chemistry R&D at Thermo Fisher Scientific
A study initiated by the International Society for the Advancement of Flow Cytometry (ISAC) found that microspheres with assigned MESF (molecules of equivalent soluble fluorophore) values were not always accurate and differed considerably from one manufacturer to another. To address these inconsistencies, the ERF (equivalent reference fluorophore) intensity unit was introduced by the National Institute of Standards and Technology (NIST).
SARS-CoV-2 Infection as a Potential Trigger for Diabetes: The Forest and The Trees
Speaker: Dirk Homann, MD, PhD, MA, Professor of Medicine at Icahn School of Medicine at Mount Sinai
The relationship between SARS-CoV-2 infection and diabetes has been proposed to exhibit a “bidirectional dynamic”, where pre-existing diabetes may exacerbate the course of COVID-19, and SARS-CoV-2 infection, in turn, may precipitate diabetes.
Streamlining and Democratizing Functional Testing by Flow Cytometry
Speaker: Jean-Marc Busnel, Ph.D. Senior Staff Research Scientist, Beckman Coulter Streamlining and Democratizing Functional Testing by Flow CytometryWebinar Topics Include: 1) Allergy testing with streamlined basophil activation testing2) Flow cytometry based...
MACSQuant® Tyto®: Plug & play cell sorting in a fully closed cartridge system
Speaker: Dr. Felix Eppler, Ph.D. Junior Product Manager, Miltenyi Biotec In this webinar, you will learn about the microchip-based sorting technology of the MACSQuant® Tyto® Cell Sorter. Find out how cell sorting within a closed cartridge system eliminates the risk of...
Conventional and Spectral Flow Cytometry Panel Building and Data Analysis with FCS Express
Speaker: Sean Burke – De Novo Software and Jeff Jensen – FluoroFinder De Novo Software and FluoroFinder are collaborating to help bridge the gap from experiment design to data analysis for both conventional and spectral flow cytometry. The one hour webinar will...
5 Most Useful Flow Cytometry Panel Design Features on FluoroFinder
Speaker: Jeff Jensen, Flow Cytometry Lead & Head of Sales, FluoroFinder One of the biggest challenges of multi-color flow cytometry is selecting the right combination of fluorochromes and antibody conjugates that fit your cytometer so that the quality of data is...
A New Age of Spectral Cytometry: 26-color B-cell Lymphopoiesis
Speaker: Kelly Lundsten, Business Segment Manager, Adv Cytometry, PD Flow The Cytek Aurora flow cytometer has made more than twenty-four color flow cytometry panels possible with only three lasers. The spectral overlap of traditional fluorophores previously limited...
Panel Design Considerations for Spectral Flow Cytometry
Speaker: Jerry Barnhart, Application Scientist, Sony Biotechnology In this webinar, we will explore the key considerations when designing multicolor panels for use on the ID7000™ spectral cell analyzer. We will review the available fluorochromes with respect to their...
Single-Cell Biology of Barrier Tissues and COVID-19
Speaker: Dr. José Ordovas-Montañes, PhD, principal investigator at Boston Children’s Hospital, assistant professor at Harvard Medical School, associate member at the Broad Institute, and principal faculty at Harvard Stem Cell Institute
In this talk, Dr. Ordovas-Montañes will discuss recent studies utilizing single-cell genomics to comprehensively map the cell types, subsets, and states that compose human barrier tissues.
Flow Cytometry, the ZE5 Cell Analyzer, and the Fight against COVID-19
Speaker: Dr. Richard Cuthbert, Flow Cytometry Specialist, Life Science Group at Bio-Rad
In this webinar, Dr Richard Cuthbert reviews how the ZE5 Cell Analyzer has been used in the fight against COVID-19. He discusses advances in the understanding of the disease and how flow cytometry is being used to develop new treatments. Finally, he highlights why Bio-Rad’s ZE5 Cell Analyzer is particularly suited for applications such as screening, antibody discovery, and vaccine development.
High Dimensional Flow Cytometric Characterization of Complex Tissues With Infinity Flow
Speaker: Mark Headley, Ph.D. Assistant Professor, Fred Hutchinson Cancer Research Center, Clinical Research Division, Program In Immunology, Affiliate Assistant Professor, University of Washington, Department of Immunology
This webinar will provide basic training in the implementation of Infinity Flow. Infinity Flow combines sophisticated machine learning approaches with parallelized flow cytometric staining to profile the coexpression patterns of hundreds of markers in tandem at single-cell resolution in complex tissue samples across both mouse and human specimens. Dr. Headley will provide background on the basic principles of Infinity Flow as well as its application to understanding complex biology in human and mouse specimens.
Quantifying T Cell Exhaustion: An Insight into BiTE Antibodies and CAR-T Cells for Improved Therapeutics
Speaker: Lauren Kelsey, Scientist, iQue Applications Group, Sartorius
Early Life Development of Thymic Microbiota-Specific T Cells
Speaker: Daniel Zegarra-Ruiz, PhD, Research Fellow at Memorial Sloan Kettering Cancer Center
In this webinar, Dr. Daniel Zegarra-Ruiz of Memorial Sloan Kettering Cancer Center, recipient of the AAI 2020 Lefrançois-BioLegend Memorial Award, discusses how thymic T cell development is altered after colonization with model intestinal microbes.
Multiplexed Functional Antibody Profiling Using Advanced Flow Cytometry
Speaker: Dr. John O’Rourke With breakthroughs in molecular engineering and antibody humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. Monoclonal antibodies are the backbone of many treatment modalities including...
CITE-Seq Identifies Novel Stromal Cell Populations in Diseased Versus Healthy Human Tendon
Speaker: Dr. Adrian Kendal Chronic tendinopathy affects over 15% of the population and represents a growing healthcare challenge in an active and increasingly aging population. The lower limbs are most commonly affected, with a clinical spectrum ranging from isolated...
An Optimized Method for Accurate Spread Measurement and its Use in Panel Design
Speakers: Dr. Mirko Corselli & Bob Balderas When designing multicolor flow cytometry panels, assessment of fluorescence spillover is critical to prevent or minimize loss of resolution due to spreading error. The Spillover Spread Matrix (SSM) was developed as a...
Apotracker™ Green (Apo-15) as a Novel Fluorescent Probe to Accelerate the Detection and Imaging of Apoptotic Cells
Speakers: Dr. Nicole Barth and Prof. Marc Vendrell In the human body, billions of cells die every day; this cell death is termed apoptosis. Clearance of these cells is crucial for the maintenance of homeostasis, and its dysregulation can lead to inflammatory diseases...
Evaluating Therapeutic Antibodies: Quantification of ADCP using Advanced Flow Cytometry
Speaker: Kristy McBain This webinar will provide an overview of advanced high throughput flow cytometry. Followed by a discussion of the mechanisms of tumor clearance by antibody dependant cellular phagocytosis (ADCP) and how it compares to other monoclonal antibody...