Webinars

On-Demand Webinars  

This collection of webinars speaks to experimental design across industries that utilize tools that FluoroFinder offers on our platform. We regularly update these webinars as an invaluable resource for our community of users. 

Optimizing Controls for Flow Cytometry

Optimizing Controls for Flow Cytometry

Speaker: Amay Dankar, M.S., Associate Director of Field Applications, Slingshot Biosciences
In this educational webinar, Amay discusses common controls that users need to run their best experiments and the prerequisites for running optimized panels.

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Novel Fluorescent and Magnetic Reagents for Life Science Research

Novel Fluorescent and Magnetic Reagents for Life Science Research

Speaker: Kristie Krug, CEO of Core Quantum Technologies
In this webinar, Kristie discusses the development and application of Core Quantum Technology’s highly stable fluorescent reagents with narrow emissions and greater tunability, as well as their unique magnetic and fluorescent reagent that allows for magnetic separation and immediate fluorescent labeling.

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Debunking Antibody Titration Myths

Debunking Antibody Titration Myths

Speaker: Patrick Duncker, Technical Lead, Cytek Biosciences.
In this webinar, we discuss the best practices for antibody titrations, metrics used to evaluate optimal titer, and the impact of flow cytometer setup and configuration on antibody titer.

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A Serum-Free, Chemically-Defined Workflow for T Cell Therapy

A Serum-Free, Chemically-Defined Workflow for T Cell Therapy

Speaker: Zayda Piedra, PhD, Product Development Scientist at BioLegend
In this webinar, we will go over current challenges in the cell therapy industry and what we can do better, BioLegend’s emerging solutions for T cell therapy – closing the gaps, one at a time, and the importance of bioprocessing reagents manufactured with consistent, high-quality well-defined components.

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Highly Multiplexed Imaging of Diverse Tissues Using IBEX

Highly Multiplexed Imaging of Diverse Tissues Using IBEX

Speaker: Andrea Radtke, Ph.D. and Dr. Ziv Yaniv, Ph.D.
In this webinar, we will go over methods to study normal and malignant lymph nodes and topics related to multiplexed tissue imaging, namely: optimal tissue processing, working with fixed frozen and FFPE specimens, overcoming autofluorescence, identifying appropriate reagents, validating antibodies, and creating organ mapping antibody panels (OMAPs).

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MA900 Cell Sorter: Increasing Application Capabilities in Multi-User Laboratories

MA900 Cell Sorter: Increasing Application Capabilities in Multi-User Laboratories

Speaker: Anthony Carcio, Field Application Scientist, Sony Biotechnology
This webinar will provide insights to researchers who are new to sorting or who operate shared flow facilities on how the Sony MA900 can support varied user needs and guided workflows for sorting. Learn how an automated and streamlined sort setup can increase the capabilities within a multi-user lab, review how the MA900 enables seamless setup and exchange of different chips without instrument downtime between users, and understand how the MA900 can be maintained in multi-user labs for successful, high-throughput, and gentle sorting of various cells.

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Evaluation of Large Antibody Panels in Single-Cell Genomic Immunophenotyping of Fresh and Frozen Human Leukocytes

Evaluation of Large Antibody Panels in Single-Cell Genomic Immunophenotyping of Fresh and Frozen Human Leukocytes

Speaker: Dr. Jasmine Ying-Jiun Chen
Dr. Chen will share Abiosciences’ effort in evaluating BioLegend’s TotalSeq™-C Human Universal Cocktail using the 10x Genomics single-cell platform on fresh and frozen PBMCs. This includes bioinformatics pipelines to compare the quality control metrics, isotype matched control performance, and cell subtype analyses. Advanced visualization and analysis tools were developed to interrogate the data in a flow cytometry paradigm, including complex gating strategies to identify rare cell subtypes. The pilot study is being expanded to larger scale clinical settings and may help characterize important cell populations that are associated with disease status, pharmacodynamics, and therapeutic response.

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Cell Painting – A Practical Approach

Cell Painting – A Practical Approach

Speaker: Joe Trask, North America HCS Product Line Leader, Cellular Imaging and Analysis, PerkinElmer, and Alexander Schreiner Team Leader, Biological Applications Team, PerkinElmer, Hamburg, Germany
Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology. Specific cellular compartments and organelles are simultaneously stained with six fluorescent probes, followed by imaging and analysis to extract hundreds to thousands of features to phenotypically profile the cells and gain a deeper understanding of the effects of chemical, drug, or genetic perturbagens.

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Measuring T Cell Exhaustion: A Multiplexed Approach Utilizing Advanced Flow Cytometry

Measuring T Cell Exhaustion: A Multiplexed Approach Utilizing Advanced Flow Cytometry

Speaker: Mark Carter, Manager of Assay Development. Product Development Cell Analytics, Sartorius
Understanding immune system dynamics and their role in health and disease, as well as developing novel therapies, relies on the use of powerful analytical screening tools to probe cellular functions. Characterizing immune cell function such as the induction and persistence of T cell exhaustion is important for furthering therapeutic interventions.

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AccuCheck ERF Reference Particles: Easy to Use Flow Cytometry Intensity Reference with Accurate NIST-Assigned Values

AccuCheck ERF Reference Particles: Easy to Use Flow Cytometry Intensity Reference with Accurate NIST-Assigned Values

Adam W. York, Ph.D. Sr. Staff Scientist in Chemistry R&D at Thermo Fisher Scientific
A study initiated by the International Society for the Advancement of Flow Cytometry (ISAC) found that microspheres with assigned MESF (molecules of equivalent soluble fluorophore) values were not always accurate and differed considerably from one manufacturer to another. To address these inconsistencies, the ERF (equivalent reference fluorophore) intensity unit was introduced by the National Institute of Standards and Technology (NIST).

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Single-Cell Biology of Barrier Tissues and COVID-19

Single-Cell Biology of Barrier Tissues and COVID-19

Speaker: Dr. José Ordovas-Montañes, PhD, principal investigator at Boston Children’s Hospital, assistant professor at Harvard Medical School, associate member at the Broad Institute, and principal faculty at Harvard Stem Cell Institute
In this talk, Dr. Ordovas-Montañes will discuss recent studies utilizing single-cell genomics to comprehensively map the cell types, subsets, and states that compose human barrier tissues.

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Flow Cytometry, the ZE5 Cell Analyzer, and the Fight against COVID-19

Flow Cytometry, the ZE5 Cell Analyzer, and the Fight against COVID-19

Speaker: Dr. Richard Cuthbert, Flow Cytometry Specialist, Life Science Group at Bio-Rad
In this webinar, Dr Richard Cuthbert reviews how the ZE5 Cell Analyzer has been used in the fight against COVID-19. He discusses advances in the understanding of the disease and how flow cytometry is being used to develop new treatments. Finally, he highlights why Bio-Rad’s ZE5 Cell Analyzer is particularly suited for applications such as screening, antibody discovery, and vaccine development.

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High Dimensional Flow Cytometric Characterization of Complex Tissues With Infinity Flow

High Dimensional Flow Cytometric Characterization of Complex Tissues With Infinity Flow

Speaker: Mark Headley, Ph.D. Assistant Professor, Fred Hutchinson Cancer Research Center, Clinical Research Division, Program In Immunology, Affiliate Assistant Professor, University of Washington, Department of Immunology
This webinar will provide basic training in the implementation of Infinity Flow. Infinity Flow combines sophisticated machine learning approaches with parallelized flow cytometric staining to profile the coexpression patterns of hundreds of markers in tandem at single-cell resolution in complex tissue samples across both mouse and human specimens. Dr. Headley will provide background on the basic principles of Infinity Flow as well as its application to understanding complex biology in human and mouse specimens.

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