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FluoroFinder News & Updates  

From flow cytometry research and experimental design trends to FluoroFinder tool updates and industry applications, we explore it all in our blog.  

Tandem Dyes

Tandem Dyes

The commercial availability of antibody-linked fluorescent dyes has expanded rapidly over the past decade. Where researchers were once limited to a handful of common dyes (FITC, PE, APC, PerCP, etc.), they can now choose from an extensive list of fluorescent dye lines...

Fluorescence Recovery After Photobleaching

Fluorescence Recovery After Photobleaching

Fluorescence recovery after photobleaching is a technique for characterizing the mobility of cellular molecules   Fluorescence recovery after photobleaching (FRAP), also known as fluorescence redistribution after photobleaching, is a microscopy-based technique...

Optimizing Your Immunophenotyping Assay

Optimizing Your Immunophenotyping Assay

Antibody conjugate panels must be designed with care to ensure accurate results Immunophenotyping is a flow cytometry-based technique which uses fluorophore-labeled antibodies for identifying different cell types within a heterogeneous population. While some cell...

Advances in Microscopy

Advances in Microscopy

Microscopy is comprised of many innovative technologies and remains at the forefront of scientific research.   Since the invention of the microscope in the late 16th century, there has been a continual push to produce instruments with higher resolution, faster...

Guide to Fixation and Permeabilization

Guide to Fixation and Permeabilization

Fixation and permeabilization are key protocol steps for several core immunoassay techniques Immunoassay techniques such as flow cytometry, immunocytochemistry (ICC), and immunohistochemistry (IHC) often require that samples be fixed and/or permeabilized prior to...

Roundup of Spectral Flow Cytometers

Roundup of Spectral Flow Cytometers

The use of spectral flow cytometry is increasing due to the many benefits it affords. Spectral flow cytometry is a relatively new technology that was developed to push the boundaries of traditional flow cytometry. First described in 2004 by researchers at Purdue...

Near-Infrared Fluorophores For Fluorescence Imaging

Near-Infrared Fluorophores For Fluorescence Imaging

Near-infrared fluorophores offer several advantages for imaging applications. A major advantage of fluorescent detection is that it enables multiple antibodies to be combined in the same experiment. Using techniques such as immunocytochemistry (ICC) and...

Fantastic Beasts and How To Image Them

Fantastic Beasts and How To Image Them

Fluorescence microscopy is seeing increased utility for imaging organisms While fluorescence microscopy has long been employed for imaging plated mammalian cells, its use for visualizing organisms is flourishing. In recent years, winning images in Nikon’s annual Small...

Preparing a Single-Cell Suspension for Flow Cytometry

Preparing a Single-Cell Suspension for Flow Cytometry

High viabilities, well-preserved antigens, and minimal clumping underpin flow cytometry success Unlike bulk population analysis techniques such as ELISA and Western blot, flow cytometry provides information about individual cells. Although microscopy does this as...

OMIPs

OMIPs

What are OMIPs?  Optimized multicolor immunofluorescence panels, commonly known as OMIPs, are highly validated sets of antibodies and reagents that are published, peer-reviewed, and compiled to serve as a resource to the scientific community. OMIP publications often...

Advances in Western Blot Technologies

Advances in Western Blot Technologies

New approaches to Western blotting techniques overcome performance limitations For over 40 years, researchers have used Western blot for protein identification. Not only does Western Blot provide confirmation that a target of interest is present in a sample, this...

Expansion Microscopy

Expansion Microscopy

Expansion Microscopy physically expands biological specimens enabling higher resolution imaging   First described in 2015, expansion microscopy is an imaging technique that improves on the resolution of conventional light microscopy by physically expanding the...

Flow Cytometry in Stem Cell Research and Therapy

Flow Cytometry in Stem Cell Research and Therapy

Identifying distinct stem cell types and monitoring their differentiation is essential for developing novel therapies   Stem cell research originated in the 1950s when it was discovered that mice which had received a lethal dose of radiation could recover...

Excluding Dead Cells With a Cell Viability Fluorescent Dye

Excluding Dead Cells With a Cell Viability Fluorescent Dye

Excluding dead cells from flow cytometry analysis with a cell viability fluorescent dye is critical for producing accurate results   The presence of dead cells in samples that will be analyzed by flow cytometry can affect antibody staining and lead to inaccurate...

Newsletter: Imaging and Analysis of 3D Cell Cultures

Newsletter: Imaging and Analysis of 3D Cell Cultures

Compared to traditional monolayers, 3D cell cultures more closely resemble physiological conditions 2-dimensional (2D) cell cultures consisting of monolayers attached to a flat surface have been a mainstay of scientific research for decades. However, cells grown in...

Newsletter: Considerations for Spectral Flow Cytometry Panel Design

Newsletter: Considerations for Spectral Flow Cytometry Panel Design

Spectral flow cytometry offers several advantages over conventional flow cytometry but may be more affected by spreading error Spectral flow cytometry was first described in 2004 by the Robinson group at Purdue University Cytometry Laboratories. Since then, several...

Newsletter: Confocal Microscopy and Related Technologies

Newsletter: Confocal Microscopy and Related Technologies

Confocal microscopy offers several advantages over traditional widefield microscopy and has evolved to meet many different research needs Originally developed by Marvin Minsky in the 1950s to address the problem of light scatter, confocal microscopy has become an...

Newsletter: Distinguishing Cell-Cell Complexes from Singlet Cells

Newsletter: Distinguishing Cell-Cell Complexes from Singlet Cells

Failure to correctly identify cell-cell complexes can lead to data being misinterpreted and compromise the purity of sorted cell populations Multiparametric flow cytometry allows researchers to detect different cell types within a heterogeneous population. When...

Newsletter: Antibody Selection Guidelines

Newsletter: Antibody Selection Guidelines

Choosing the right antibody for the intended application and sample type is critical to producing reliable results Antibodies are essential tools for applications ranging from flow cytometry, immunocytochemistry (ICC), and immunohistochemistry (IHC) to Western...

Newsletter: Comparison of Flow Cytometry Analysis Software

Newsletter: Comparison of Flow Cytometry Analysis Software

Software packages for viewing and analyzing FCS data files offer different features and benefits Data analysis represents a major source of variation in flow cytometry. Not only is the analysis process highly prone to user subjectivity, but disparities may arise due...

Newsletter: Fluorescent proteins: advantages and disadvantages

Newsletter: Fluorescent proteins: advantages and disadvantages

Fluorescent proteins have enabled countless scientific discoveries, but they have their limitations For decades, researchers have been fascinated by the ability of certain living organisms to emit light. However, it was not until 1962 that this phenomenon was first...

Newsletter: CRISPR as an Imaging Tool

Newsletter: CRISPR as an Imaging Tool

Repurposing the CRISPR-Cas system for imaging genomic targets in living cells Established methods for cell imaging use fluorophore-labeled antibodies or oligonucleotides to bind proteins or nucleic acids in fixed cells or rely on the engineered expression of...

Newsletter: Past, Present, and Future of Flow Cytometry

Newsletter: Past, Present, and Future of Flow Cytometry

Flow cytometry continues to evolve with advances in instrumentation, reagents, and software The first fluorescence-based flow cytometer was developed in 1968 and was commercialized the following year. Since then, the field of flow cytometry has matured rapidly, and...

Newsletter: Fluorescent Probes for Intracellular Calcium Measurement

Newsletter: Fluorescent Probes for Intracellular Calcium Measurement

Studying calcium flux leads to insights into normal physiology and disease Calcium ions (Ca2+) are involved in numerous intracellular signaling cascades and in a broad range of physiological processes. These include neurotransmission, muscle contraction, and hormone...

Newsletter: Cellular Markers for Flow Cytometry Analysis

Newsletter: Cellular Markers for Flow Cytometry Analysis

Marker selection and panel design underpin the accuracy of cellular identification Being able to monitor the presence and relative abundance of different cell types in sample material is essential to understand normal biology and investigate the pathogenesis of...

Newsletter: Dyes for Analyzing the Cell Cycle and Apoptosis

Newsletter: Dyes for Analyzing the Cell Cycle and Apoptosis

Aberrant cell proliferation and cell death underlie a multitude of disease states Normal tissue homeostasis depends on a critical balance between cell proliferation and cell death. The cell cycle regulates the former, while the latter occurs via controlled...

Newsletter: Fluorophores for Confocal Microscopy

Newsletter: Fluorophores for Confocal Microscopy

 Confocal microscopy is a powerful imaging technique used to study biological specimens. It offers several advantages over conventional widefield microscopy, including the capacity to control depth-of-field and collect serial sections from thick samples. Fluorophores...

Newsletter: Designing a Fluorescence Microscopy Experiment

Newsletter: Designing a Fluorescence Microscopy Experiment

For over 100 years, researchers have used fluorescence microscopy to study biological samples like plated cells or tissue sections. Such experiments typically involve using fluorophore-labeled antibodies to recognize and bind targets of interest before imaging with a...

Newsletter: Analysis of Rare Cell Populations

Newsletter: Analysis of Rare Cell Populations

Rare cell types provide novel insights into mechanisms of health and disease Many techniques used for cell-based research rely on bulk analysis, with data being generated from a complex heterogeneous population. The main drawback of these methods is that they can lead...

Newsletter: Overview of Advanced Fluorescence Microscopy Techniques

Newsletter: Overview of Advanced Fluorescence Microscopy Techniques

Conventional fluorescence microscopy uses fluorophore-labeled antibodies to visualize cellular structures, providing insights into cellular physiological and pathological states. However, the wave nature of light imposes a resolution limit of approximately 200 nm on...

Newsletter: Single-Cell Analysis in Immunology

Newsletter: Single-Cell Analysis in Immunology

In recent years, technologies for single-cell analysis have evolved rapidly, revealing huge differences between cells once categorized as being the same type. This variability is most apparent within the cells of the immune system, which must undergo multiple dynamic...

Newsletter: OMIP Resource and Validated Panel

Newsletter: OMIP Resource and Validated Panel

The “optimized multicolor immunofluorescence panel” OMIP publication format was launched 11 years ago as a collaborative platform to establish criteria for experimental design, data collection, and analysis [1]. An OMIP is, by definition, a peer-reviewed publication...

Newsletter: Imaging Flow Cytometry

Newsletter: Imaging Flow Cytometry

A technique that combines flow cytometry with digital microscopy promises novel insights Imaging flow cytometry (IFC) has seen increased uptake in recent years, largely due to instrumentation and software improvements that have made the technique more accessible. By...

Newsletter: History of fluorescent dyes

Newsletter: History of fluorescent dyes

The widespread adoption of fluorescence applications is a testament to the importance of fluorescent dyes in biochemical and biological research. The speed, reproducibility, and sensitivity of fluorescence-based analysis methods explain their rapid adoption in all...

Newsletter: Essential Flow Cytometry Bookmarks

Newsletter: Essential Flow Cytometry Bookmarks

Typing “flow cytometry” into the Google search box returns 11,300,000 results! A daunting amount of information to process for anyone approaching the technology for the first time. To help anyone wanting to learn more about flow cytometry navigate this huge body of...

Newsletter: Spectral Flow Cytometry

Newsletter: Spectral Flow Cytometry

The recent publication of a 43 color panel for the characterization of Peripheral Blood Mononuclear Cells (PBMC) is yet another example of the multiplexing potential of spectral flow cytometry. The technology, first demonstrated by Dr. Paul Robinson at Purdue...

Newsletter: Reducing Background Signal with Recombinant Antibodies

Newsletter: Reducing Background Signal with Recombinant Antibodies

Enhance the quality of your flow cytometry data by using recombinant antibodies Recombinant antibodies have many advantages over conventional antibody reagents. These include exceptional batch-to-batch reproducibility, highly scalable in vitro production, and the...

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