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FluoroFinder News & Updates  

From flow cytometry research and experimental design trends to FluoroFinder tool updates and industry applications, we explore it all in our blog.  

Newsletter: Confocal Microscopy and Related Technologies

Newsletter: Confocal Microscopy and Related Technologies

Confocal microscopy offers several advantages over traditional widefield microscopy and has evolved to meet many different research needs Originally developed by Marvin Minsky in the 1950s to address the problem of light scatter, confocal microscopy has become an...

Newsletter: Distinguishing Cell-Cell Complexes from Singlet Cells

Newsletter: Distinguishing Cell-Cell Complexes from Singlet Cells

Failure to correctly identify cell-cell complexes can lead to data being misinterpreted and compromise the purity of sorted cell populations Multiparametric flow cytometry allows researchers to detect different cell types within a heterogeneous population. When...

Newsletter: Antibody Selection Guidelines

Newsletter: Antibody Selection Guidelines

Choosing the right antibody for the intended application and sample type is critical to producing reliable results Antibodies are essential tools for applications ranging from flow cytometry, immunocytochemistry (ICC), and immunohistochemistry (IHC) to Western...

Newsletter: Comparison of Flow Cytometry Analysis Software

Newsletter: Comparison of Flow Cytometry Analysis Software

Software packages for viewing and analyzing FCS data files offer different features and benefits Data analysis represents a major source of variation in flow cytometry. Not only is the analysis process highly prone to user subjectivity, but disparities may arise due...

Newsletter: Fluorescent proteins: advantages and disadvantages

Newsletter: Fluorescent proteins: advantages and disadvantages

Fluorescent proteins have enabled countless scientific discoveries, but they have their limitations For decades, researchers have been fascinated by the ability of certain living organisms to emit light. However, it was not until 1962 that this phenomenon was first...

Newsletter: CRISPR as an Imaging Tool

Newsletter: CRISPR as an Imaging Tool

Repurposing the CRISPR-Cas system for imaging genomic targets in living cells Established methods for cell imaging use fluorophore-labeled antibodies or oligonucleotides to bind proteins or nucleic acids in fixed cells or rely on the engineered expression of...

Newsletter: Past, Present, and Future of Flow Cytometry

Newsletter: Past, Present, and Future of Flow Cytometry

Flow cytometry continues to evolve with advances in instrumentation, reagents, and software The first fluorescence-based flow cytometer was developed in 1968 and was commercialized the following year. Since then, the field of flow cytometry has matured rapidly, and...

Newsletter: Cellular Markers for Flow Cytometry Analysis

Newsletter: Cellular Markers for Flow Cytometry Analysis

Marker selection and panel design underpin the accuracy of cellular identification Being able to monitor the presence and relative abundance of different cell types in sample material is essential to understand normal biology and investigate the pathogenesis of...

Newsletter: Dyes for Analyzing the Cell Cycle and Apoptosis

Newsletter: Dyes for Analyzing the Cell Cycle and Apoptosis

Aberrant cell proliferation and cell death underlie a multitude of disease states Normal tissue homeostasis depends on a critical balance between cell proliferation and cell death. The cell cycle regulates the former, while the latter occurs via controlled...

Newsletter: Fluorophores for Confocal Microscopy

Newsletter: Fluorophores for Confocal Microscopy

 Confocal microscopy is a powerful imaging technique used to study biological specimens. It offers several advantages over conventional widefield microscopy, including the capacity to control depth-of-field and collect serial sections from thick samples. Fluorophores...

Newsletter: Designing a Fluorescence Microscopy Experiment

Newsletter: Designing a Fluorescence Microscopy Experiment

For over 100 years, researchers have used fluorescence microscopy to study biological samples like plated cells or tissue sections. Such experiments typically involve using fluorophore-labeled antibodies to recognize and bind targets of interest before imaging with a...

Newsletter: Analysis of Rare Cell Populations

Newsletter: Analysis of Rare Cell Populations

Rare cell types provide novel insights into mechanisms of health and disease Many techniques used for cell-based research rely on bulk analysis, with data being generated from a complex heterogeneous population. The main drawback of these methods is that they can lead...

Newsletter: Overview of Advanced Fluorescence Microscopy Techniques

Newsletter: Overview of Advanced Fluorescence Microscopy Techniques

Conventional fluorescence microscopy uses fluorophore-labeled antibodies to visualize cellular structures, providing insights into cellular physiological and pathological states. However, the wave nature of light imposes a resolution limit of approximately 200 nm on...

Newsletter: Single-Cell Analysis in Immunology

Newsletter: Single-Cell Analysis in Immunology

In recent years, technologies for single-cell analysis have evolved rapidly, revealing huge differences between cells once categorized as being the same type. This variability is most apparent within the cells of the immune system, which must undergo multiple dynamic...

Newsletter: OMIP Resource and Validated Panel

Newsletter: OMIP Resource and Validated Panel

The “optimized multicolor immunofluorescence panel” OMIP publication format was launched 11 years ago as a collaborative platform to establish criteria for experimental design, data collection, and analysis [1]. An OMIP is, by definition, a peer-reviewed publication...

Newsletter: Imaging Flow Cytometry

Newsletter: Imaging Flow Cytometry

A technique that combines flow cytometry with digital microscopy promises novel insights Imaging flow cytometry (IFC) has seen increased uptake in recent years, largely due to instrumentation and software improvements that have made the technique more accessible. By...

Newsletter: History of fluorescent dyes

Newsletter: History of fluorescent dyes

The widespread adoption of fluorescence applications is a testament to the importance of fluorescent dyes in biochemical and biological research. The speed, reproducibility, and sensitivity of fluorescence-based analysis methods explain their rapid adoption in all...

Newsletter: Essential Flow Cytometry Bookmarks

Newsletter: Essential Flow Cytometry Bookmarks

Typing “flow cytometry” into the Google search box returns 11,300,000 results! A daunting amount of information to process for anyone approaching the technology for the first time. To help anyone wanting to learn more about flow cytometry navigate this huge body of...

Newsletter: Spectral Flow Cytometry

Newsletter: Spectral Flow Cytometry

The recent publication of a 43 color panel for the characterization of Peripheral Blood Mononuclear Cells (PBMC) is yet another example of the multiplexing potential of spectral flow cytometry. The technology, first demonstrated by Dr. Paul Robinson at Purdue...

Newsletter: Reducing Background Signal with Recombinant Antibodies

Newsletter: Reducing Background Signal with Recombinant Antibodies

Enhance the quality of your flow cytometry data by using recombinant antibodies Recombinant antibodies have many advantages over conventional antibody reagents. These include exceptional batch-to-batch reproducibility, highly scalable in vitro production, and the...

Newsletter: Fluorophores for Super-Resolution Microscopy

Newsletter: Fluorophores for Super-Resolution Microscopy

Breaking through the diffraction barrier for deeper biological insights Super-resolution microscopy (SRM) improves conventional fluorescence microscopy by achieving higher resolution. It enables fine mapping of structures such as microtubule protofilaments,...

Newsletter: Gating Strategies

Newsletter: Gating Strategies

Analysis of flow cytometry data requires the identification of a homogenous cell population. This is achieved in a multistep process known as gating. It involves narrowing down the parameters that define the cell population of interest and excluding dead cells and...

Newsletter: Dyes for Spectral Analyzers

Newsletter: Dyes for Spectral Analyzers

Maximize the benefits of spectral flow cytometry by using the right fluorochromes Spectral flow cytometry enables researchers to conduct larger and more comprehensive flow cytometry experiments than ever before, in some cases with over 40 different fluorochromes in a...

Newsletter: New Technology Roundup

Newsletter: New Technology Roundup

Some of the latest developments advancing scientific research With 2021 now well underway, we felt it might be timely to highlight some of the newer technologies available to enhance laboratory workflows. Modern dyes designed to increase panel size have proven hugely...

Newsletter: Tips and Tools for Flow Cytometry Sample Prep Success

Newsletter: Tips and Tools for Flow Cytometry Sample Prep Success

Improve your flow cytometry sample prep for more reliable results Sample material is precious, so it’s essential that flow cytometry data is both reliable and reproducible. This means optimizing every step of your flow cytometry protocol, beginning with sample prep,...

Introducing FluoroFinder’s Fluorescent Dye Directory

Introducing FluoroFinder’s Fluorescent Dye Directory

Introducing FluoroFinder’s Fluorescent Dye Directory Innovations in fluorescent dye technology coupled with increased demand for more efficient research tools have resulted in the development of an incredible amount of new fluorescent dye products in recent years....

Newsletter: Intracellular Flow Cytometry Fundamentals

Newsletter: Intracellular Flow Cytometry Fundamentals

Intracellular Flow Cytometry Fundamentals Proteins produced by cells get shuttled around and end up in a variety of locations, whether they are embedded on the cell surface, contained internally within the cell, or secreted outside of the cell. Intracellular flow...

Newsletter: Live-Cell 3D Imaging with Fluorescence Nanoscopy

Newsletter: Live-Cell 3D Imaging with Fluorescence Nanoscopy

Live-Cell 3D Imaging with Fluorescence Nanoscopy Advances in microscopy techniques now allow for ultra-high-resolution imaging of fluorescently labeled markers within living three-dimensional (3D) cell cultures. This provides researchers with a more accurate spatial...

Newsletter: Antigen Density: Why Brighter Is Not Always Better

Newsletter: Antigen Density: Why Brighter Is Not Always Better

Antigen Density Antigen density refers to the number of target molecules in a specific cell subset.  Antigens expressed at high levels are densely populated on the cell, making them more readily available for antibody detection.  Understanding antigen density is...

Newsletter: Flow Cytometry Fights COVID-19

Newsletter: Flow Cytometry Fights COVID-19

How  cytometry  is  helping researchers  fight  a pandemic Flow cytometry is playing a central role in shaping our understanding of COVID-19 disease and the SARS-CoV-2 virus. Here we highlight how research teams are using flow cytometry to detect SARS-CoV-2, identify...

Newsletter: State of Spectral Flow Cytometry

Newsletter: State of Spectral Flow Cytometry

State of Spectral Flow Cytometry Spectral analyzers are the next generation of multicolor flow cytometers offering unparalleled sensitivity, increased detection range, do-it-all flexibility, and ease of use. Unlike conventional flow cytometers which use dichroic...

Newsletter: Choosing a Flow Cytometry Analysis Software

Newsletter: Choosing a Flow Cytometry Analysis Software

Analysis Software   FluoroFinder’s panel builder tool is the obvious choice to simplify your flow cytometry experiment design, but you will also need to choose software for efficient data analysis. Your lab or core facility may already have one or more analysis...

Newsletter: Flow Cytometry Applications for Blood Immunophenotyping

Newsletter: Flow Cytometry Applications for Blood Immunophenotyping

Flow Cytometry Applications for Blood Immunophenotyping Immunophenotyping refers to the process of detecting expressed protein markers to identify and quantify specific subsets of cells within a heterogeneous population.  Flow cytometry is the most common method of...

Newsletter: Tandem Dyes – Life is Brighter with a Buddy

Newsletter: Tandem Dyes – Life is Brighter with a Buddy

Tandem Dyes The commercial availability of antibody-linked fluorescent dyes has expanded rapidly over the past decade. Where researchers were once limited to a handful of common dyes (FITC, PE, APC, PerCP, etc.), they can now choose from an extensive list of...

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