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FluoroFinder News & Updates  

From flow cytometry research and experimental design trends to FluoroFinder tool updates and industry applications, we explore it all in our blog.  

Newsletter: History of fluorescent dyes

Newsletter: History of fluorescent dyes

The widespread adoption of fluorescence applications is a testament to the importance of fluorescent dyes in biochemical and biological research. The speed, reproducibility, and sensitivity of fluorescence-based analysis methods explain their rapid adoption in all...

Newsletter: Essential Flow Cytometry Bookmarks

Newsletter: Essential Flow Cytometry Bookmarks

Typing “flow cytometry” into the Google search box returns 11,300,000 results! A daunting amount of information to process for anyone approaching the technology for the first time. To help anyone wanting to learn more about flow cytometry navigate this huge body of...

Newsletter: Spectral Flow Cytometry

Newsletter: Spectral Flow Cytometry

The recent publication of a 43 color panel for the characterization of Peripheral Blood Mononuclear Cells (PBMC) is yet another example of the multiplexing potential of spectral flow cytometry. The technology, first demonstrated by Dr. Paul Robinson at Purdue...

Newsletter: Reducing Background Signal with Recombinant Antibodies

Newsletter: Reducing Background Signal with Recombinant Antibodies

Enhance the quality of your flow cytometry data by using recombinant antibodies Recombinant antibodies have many advantages over conventional antibody reagents. These include exceptional batch-to-batch reproducibility, highly scalable in vitro production, and the...

Newsletter: Fluorophores for Super-Resolution Microscopy

Newsletter: Fluorophores for Super-Resolution Microscopy

Breaking through the diffraction barrier for deeper biological insights Super-resolution microscopy (SRM) improves conventional fluorescence microscopy by achieving higher resolution. It enables fine mapping of structures such as microtubule protofilaments,...

Newsletter: Gating Strategies

Newsletter: Gating Strategies

Analysis of flow cytometry data requires the identification of a homogenous cell population. This is achieved in a multistep process known as gating. It involves narrowing down the parameters that define the cell population of interest and excluding dead cells and...

Newsletter: Dyes for Spectral Analyzers

Newsletter: Dyes for Spectral Analyzers

Maximize the benefits of spectral flow cytometry by using the right fluorochromes Spectral flow cytometry enables researchers to conduct larger and more comprehensive flow cytometry experiments than ever before, in some cases with over 40 different fluorochromes in a...

Newsletter: New Technology Roundup

Newsletter: New Technology Roundup

Some of the latest developments advancing scientific research With 2021 now well underway, we felt it might be timely to highlight some of the newer technologies available to enhance laboratory workflows. Modern dyes designed to increase panel size have proven hugely...

Newsletter: Tips and Tools for Flow Cytometry Sample Prep Success

Newsletter: Tips and Tools for Flow Cytometry Sample Prep Success

Improve your flow cytometry sample prep for more reliable results Sample material is precious, so it’s essential that flow cytometry data is both reliable and reproducible. This means optimizing every step of your flow cytometry protocol, beginning with sample prep,...

Introducing FluoroFinder’s Fluorescent Dye Directory

Introducing FluoroFinder’s Fluorescent Dye Directory

Introducing FluoroFinder’s Fluorescent Dye Directory Innovations in fluorescent dye technology coupled with increased demand for more efficient research tools have resulted in the development of an incredible amount of new fluorescent dye products in recent years....

Newsletter: Intracellular Flow Cytometry Fundamentals

Newsletter: Intracellular Flow Cytometry Fundamentals

Intracellular Flow Cytometry Fundamentals Proteins produced by cells get shuttled around and end up in a variety of locations, whether they are embedded on the cell surface, contained internally within the cell, or secreted outside of the cell. Intracellular flow...

Newsletter: Live-Cell 3D Imaging with Fluorescence Nanoscopy

Newsletter: Live-Cell 3D Imaging with Fluorescence Nanoscopy

Live-Cell 3D Imaging with Fluorescence Nanoscopy Advances in microscopy techniques now allow for ultra-high-resolution imaging of fluorescently labeled markers within living three-dimensional (3D) cell cultures. This provides researchers with a more accurate spatial...

Newsletter: Antigen Density: Why Brighter Is Not Always Better

Newsletter: Antigen Density: Why Brighter Is Not Always Better

Antigen Density Antigen density refers to the number of target molecules in a specific cell subset.  Antigens expressed at high levels are densely populated on the cell, making them more readily available for antibody detection.  Understanding antigen density is...

Newsletter: Flow Cytometry Fights COVID-19

Newsletter: Flow Cytometry Fights COVID-19

How  cytometry  is  helping researchers  fight  a pandemic Flow cytometry is playing a central role in shaping our understanding of COVID-19 disease and the SARS-CoV-2 virus. Here we highlight how research teams are using flow cytometry to detect SARS-CoV-2, identify...

Newsletter: State of Spectral Flow Cytometry

Newsletter: State of Spectral Flow Cytometry

State of Spectral Flow Cytometry Spectral analyzers are the next generation of multicolor flow cytometers offering unparalleled sensitivity, increased detection range, do-it-all flexibility, and ease of use. Unlike conventional flow cytometers which use dichroic...

Newsletter: Choosing a Flow Cytometry Analysis Software

Newsletter: Choosing a Flow Cytometry Analysis Software

Analysis Software   FluoroFinder’s panel builder tool is the obvious choice to simplify your flow cytometry experiment design, but you will also need to choose software for efficient data analysis. Your lab or core facility may already have one or more analysis...

Newsletter: Flow Cytometry Applications for Blood Immunophenotyping

Newsletter: Flow Cytometry Applications for Blood Immunophenotyping

Flow Cytometry Applications for Blood Immunophenotyping Immunophenotyping refers to the process of detecting expressed protein markers to identify and quantify specific subsets of cells within a heterogeneous population.  Flow cytometry is the most common method of...

Newsletter: Tandem Dyes – Life is Brighter with a Buddy

Newsletter: Tandem Dyes – Life is Brighter with a Buddy

Tandem Dyes The commercial availability of antibody-linked fluorescent dyes has expanded rapidly over the past decade. Where researchers were once limited to a handful of common dyes (FITC, PE, APC, PerCP, etc.), they can now choose from an extensive list of...

Newsletters: Flow Virometry

Newsletters: Flow Virometry

Flow Virometry Multicolor flow cytometry can be used to detect and characterize viral antigens. This technique, called flow virometry, allows researchers to define viral particle profiles and better understand the relationship between infectivity and transmissibility...

Newsletter: The Potential and Limitations of Image Flow Cytometry

Newsletter: The Potential and Limitations of Image Flow Cytometry

Image Flow Cytometry Image flow cytometry (IFC) is a technique that combines fluorescent immunostaining and microfluidics with powerful digital imaging software. Like flow cytometers, image cytometers analyze heterogeneous cell populations across multiple parameters...

Newsletter: Fighting the Replication Crisis with Open Research

Newsletter: Fighting the Replication Crisis with Open Research

The replication crisis, or data irreproducibility crisis, is now a well-established problem across all scientific disciplines. This inability for researchers to replicate published experimental results exposes data integrity issues and risks potentially faulty...

Newsletter: Cytometer Instrument Types

Newsletter: Cytometer Instrument Types

Flow Cytometer/Cell Sorter Instrument Types Flow cytometers and cell sorters are two powerful tools for the accurate and efficient characterization of cells.  Both instrument types measure forward scatter (FSC), side scatter (SSC) and multiple fluorescent parameters...

Newsletter: Understanding Fluorochromes for Flow Cytometry

Newsletter: Understanding Fluorochromes for Flow Cytometry

Advances in flow cytometry instrumentation, technology and chemistries have enabled researchers to expand their experimental parameters rapidly. Experiments that were once limited to the detection of three to four parameters have increased to detect up to 40 compounds...

Newsletter: Fluorescent Protein Expression

Newsletter: Fluorescent Protein Expression

Fluorescent proteins are an important part of any molecular biologist’s toolkit. Recombinant DNA techniques allow researchers to incorporate a fluorescent protein into an engineered plasmid and track its expression over time with flow cytometry or, more commonly,...

Newsletter: FLISA vs Fluorescent ELISA

Newsletter: FLISA vs Fluorescent ELISA

The enzyme-linked immunosorbent assay (ELISA) offers a simple and effective method for rapid biomarker detection.  The main benefits of ELISA include good specificity, low cost, and straightforward readout.  However, the limited sensitivity of standard colorimetric...

Newsletter: Background Fluorescence

Newsletter: Background Fluorescence

Background fluorescence includes any signal detected beyond what is generated by the fluorochromes being measured.  The three major sources of background fluorescence include autofluorescence, spectral overlap, and undesirable antibody binding. Because false signals...

Newsletter: Advances in Flow Cytometry

Newsletter: Advances in Flow Cytometry

The field of flow cytometry continues to progress toward the goal of collecting and analyzing more data. Here we explore two recent advancements: spectral analyzer platforms to collect more data, and machine learning algorithms to analyze larger datasets. Machine...

Newsletter: Getting more from less with multiplex IHC

Newsletter: Getting more from less with multiplex IHC

Pathologists and life science researchers are increasingly performing multiplexed assays on formalin-fixed, paraffin-embedded samples.  This allows for the collection of more data from a single tissue specimen, far beyond the typical single-color immunohistochemistry...

Newsletter: The Do’s and Don’ts of Compensation

Newsletter: The Do’s and Don’ts of Compensation

Compensation is the process of correcting for spillover when one fluorophore is detected in multiple channels.  It is required for most experiments of four or more colors to identify the correct signal that should be measured in each channel.  This quick list can help...

Newsletter: Staining Index

Newsletter: Staining Index

When designing a flow cytometry experiment, it is important to consider the relative brightness of each fluorescent label on your specific instrument.  Generally, it is best to assign brighter fluorochromes to weakly expressed markers, and dimmer labels to strongly...

Newsletter: Multicolor Microscopy

Newsletter: Multicolor Microscopy

The inability to resolve signals with overlapping color spectra has traditionally limited fluorescence microscopy to a “color-barrier” of 5 colors. However innovative new developments in microscopy techniques and analysis software are allowing researchers to resolve...

Newsletter: A guide to Flow Cytometry Cell Cycle Analysis

Newsletter: A guide to Flow Cytometry Cell Cycle Analysis

Flow cytometry cell cycle analysis typically involves using a DNA binding dye to determine each cell’s total DNA content. Sorting cells by cell cycle phase is then possible, as the total DNA content fluctuates as cells pass through G0/G1, S, and G2/M phases. Cells in...

Improve your Gating Technique

Improve your Gating Technique

The first step to any successful flow cytometry data analysis is proper gating. For a complete overview of gating terminology, types and strategies, check out our previous Gating Newsletter. Here we outline 6 questions that cytometry researchers should ask themselves...

Newsletter: Apoptosis

Newsletter: Apoptosis

Flow cytometry remains the most powerful tool for studying apoptosis in cell populations. However, many researchers may not be aware of the variety of options available for apoptosis markers. Here we explore the use of flow cytometry in apoptosis research and examine...

Newsletter: Getting the most out of your cytometer

Newsletter: Getting the most out of your cytometer

Cytometer technology is continually improving, with more lasers, detectors, and channels to allow the measurement of more parameters. But researchers are too often reluctant to expand the scope of their experiments for fear of increasing design complexity. This month...

Newsletter: Antigen Density Explained

Newsletter: Antigen Density Explained

This month we explain why antigen density is important to flow cytometry studies, describe how it is measured, and list some useful references for known antigen expression densities... 1. Why Antigen Density Matters 2. Methods for Determining Antigen Density 3....

Newsletter: 7 Steps of a Successful Flow Cytometry Experiment

Newsletter: 7 Steps of a Successful Flow Cytometry Experiment

Getting started in flow cytometry may seem overwhelming, so we’ve broken the flow cytometry experiment down into seven basic steps. 1. Select your Cytometer 2. Design your panel 3. Optimizing your staining protocol                                                 4....

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