Cell Cycle

A Guide to Flow Cytometry Cell Cycle Analysis

Flow cytometry cell cycle analysis typically involves using a DNA binding dye to determine each cell’s total DNA content. Sorting cells by cell cycle phase is then possible, as the total DNA content fluctuates as cells pass through G0/G1, S, and G2/M phases…

Posted March 5, 2019


Six Simple Questions to Improve your Gating Technique

The first step to any successful flow cytometry data analysis is proper gating.Here we outline 6 questions that cytometry researchers should ask themselves to help improve your gating techniques.

Posted February 5, 2019

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2018 Trends on Panel Design

We’ve analyzed millions of data points over the past year to identify trends and opportunities in panel design. Find out if your lab is on the cutting edge or falling behind in our 2018 Trends in Fluorescent Experiment Design report.

Posted January 3, 2019


Finding the Best Dyes for Apoptosis Markers

Explore the use of flow cytometry in apoptosis research and examine various fluorescent apoptosis markers.

Posted December 5, 2018

Getting more out of your Cytometer

Are you getting the most out of your cytometer?

This month we explore how incorporating additional fluorochromes into your experiment can provide valuable data without making your panel design unnecessarily complex.

Posted November 2, 2018


Antigen Density Explained

This month we explain why antigen density is important to flow cytometry studies, describe how it is measured, and list some useful references for known antigen expression densities…

Posted October 2, 2018


7 Tips for a Successful Flow Cytometry Experiment

Getting started in flow cytometry may seem overwhelming, so we’ve broken the flow cytometry experiment down into seven basic steps…

Posted September 4, 2018

History Book

18 Facts About the History of Flow Cytometry

Did you know that Flow Cytometry was originally called ‘pulse spectro-cytophotometry’ or that the first flow cytometer was built to detect WMDs? Learn more by reading the full article…

Posted August 7, 2018


There's Nothing to FRET about Tandem Dyes

Researchers can now use several tandem dyes to improve the fluorescent signal for their experiments. However, while tandem dyes can serve as powerful research tools, they can also introduce a new set of problems to consider. Here we explore the benefits of tandem dyes and review some of the difficulties that they present.

Posted July 10, 2018


Comprehensive Guide to Compensation

Does compensation leave you feeling incompetent?

Check out this “comprehensive” list of compensation guidelines and best practices, gathered from our from our flow community, to help improve your analysis.

Posted June 5, 2018


Join the Fight Against the Reproducibility Crisis

The “reproducibility crisis”, or the inability for researchers reproduce findings, remains a growing concern for a wide range of scientific disciplines. With this article, we aim to highlight the various efforts being taken by some of our partner reagent suppliers to help combat irreproducible experimental results.

Posted May 4, 2018

7 Tips to Help You Get Published

7 Tips to Help Get Your Flow Data Published

You put a lot of time and effort into your flow cytometry experiments. Follow these 7 tips to ensure that your flow data is high-quality and more likely to be published!

Posted Apr 3, 2018


OMIPs: Stop Reinventing the Wheel!

OMIPs help simplify panel design by serving as a useful starting point for new research. In this article, we review the status of the 44 currently approved OMIPs and explain how new OMIPs are being developed with the help of FluoroFinder’s panel builder tool.

Posted Mar 6, 2018


Don't Dump the Dump Channel

This month we explore the value of using dump channels and explain how they are best utilized to improve your flow data.

Posted Feb 6, 2018


A Guide to Choosing the Right Viability Dye

This guide helps to explain the various types of viability dyes, explore their respective benefits, and summarize the commercially available dyes for each group.

Posted Jan 9, 2018

2017 Panel Design Trends Report

Analysis of Trends in Fluorescent Experiment Design

Find out if your lab is on the cutting edge or falling behind the times in our 2017 Trends in Fluorescent Experiment Design report.

Posted December 12, 2017


The Art of Flow Cytometry Gating

Flow cytometry gating can often seem like a daunting task. While there is no single solution, experienced cytometrists can recommend several tips beyond “praying for good data”. Here we define gating, explore gating methodologies, and provide some useful tips for properly gating your cells.

Posted November 14, 2017


Expand Your Experimental Palette

Flow cytometry research is continually trending toward larger panels with more colors.  This month we explore navigating the ever expanding selection of commercially available fluorescent antibodies and how researchers are getting better data by designing more colorful panels.

Posted October 10, 2017


Report: Best Practices in Panel Design

FluoroFinder has now integrated cytometer configurations for over 500 cores! With so many researchers using FluoroFinder to design their flow cytometry experiments, we wanted to share some of the excellent feedback we have received.

Posted September 12, 2017


Get More From Your Flow Core

Aside from costing your lab time and grant money, shared cytometers are a valuable resource so booking additional time may be difficult. Therefore, we have compiled this list of tips for maximizing your productivity during your time at the flow core.

Posted August 15, 2017


Cytometer Selection Made Easy

This resource page compiles information on the various commercially available cytometer platforms, including basic features and user reviews, to help you choose the best cytometer for your needs.

Posted July 11, 2017


Addressing the Reproducibility Crisis Through Better Experiment Design

FluoroFinder recognizes the importance of collaboration for good experimental design, and we are helping to fight the reproducibility crisis by maximizing the available information for each antibody in our database.

Posted June 13, 2017


Avoiding Common Mistakes in
Panel Design & Analysis

We have compiled tips to void the 6 most common pitfalls of cytometry experiment design and analysis. Learn how to avoid failed experiments, save time and frustration and get better data!

Posted May 9, 2017


Flow Cytometry Dictionary

Flow cytometry can often seem complicated and intimidating. Whether you are new to flow or an experienced researcher considering a new technique, we have compiled a glossary of important terms to help!

Posted April 10, 2017


Your Guide to Understanding Flow Cytometry Beads

Immunobeads are useful for a variety of flow cytometry related applications. Here we outline the most commonly used bead types and explain how they can be used for cytometer calibration, compensation determination and even cell sorting.

Posted March 7, 2017


Report: 2016 Trends in Panel Design

Is your lab on the cutting edge or falling behind the curve? We have analyzed thousands of panels to find these interesting trends in fluorescent experiment design.

Posted February 10, 2017


Your Essential Pre-Sort Checklist

Are you planning to run a cell sort on a precious sample? Concerned about recovery? Be sure to review our pre-sort checklist of 7 things experienced cytometrists recommend to improve cell recovery.

Posted January 10, 2017


Tracking Fluorescent Proteins

Are you designing multicolor panels around your fluorescent proteins? Would seeing your fluorescent proteins and reagents in a multi-vendor spectra viewer help you better anticipate issues?

Posted December 8, 2016


Jumpstart Your Panel Design with OMIPs

Designing a multicolor fluorescent panel can take months to develop and optimize. Fortunately, Optimized Multicolor Immunofluorescence Panels(OMIPs) have greatly reduced this process.

Posted November 8, 2016


Designing Better Panels with Antibody Validation Data

Could a new product improve your experiment? Has that new antibody been vetted by reliable testing? Linking reagent publication histories to antibodies data gives researchers more reliable info during experiment design!

Posted September 8, 2016


Viability Dyes – A Control You Can’t Live Without

Viability dyes enable you to distinguish between live and dead cell populations in your analysis. Learn why this control is critical to most flow cytometry experiments.

Posted June 30, 2016