Choosing the right antibody for the intended application and sample type is critical to producing reliable results Antibodies are essential tools for applications ranging from flow cytometry, immunocytochemistry (ICC), and immunohistochemistry (IHC) to Western...
Software packages for viewing and analyzing FCS data files offer different features and benefits Data analysis represents a major source of variation in flow cytometry. Not only is the analysis process highly prone to user subjectivity, but disparities may arise due...
Fluorescent proteins have enabled countless scientific discoveries, but they have their limitations For decades, researchers have been fascinated by the ability of certain living organisms to emit light. However, it was not until 1962 that this phenomenon was first...
Repurposing the CRISPR-Cas system for imaging genomic targets in living cells Established methods for cell imaging use fluorophore-labeled antibodies or oligonucleotides to bind proteins or nucleic acids in fixed cells or rely on the engineered expression of...
Compensation is one of the most important but least understood aspects of multicolor flow cytometry Multicolor flow cytometry allows researchers to characterize complex cellular populations by staining for several cell type specific markers at the same time. However,...
Advantages of TSA include increased sensitivity, simplified panel design, and faster time to results Tyramide signal amplification (TSA), also known as catalyzed reporter deposition (CARD) or tyramine amplification technique (TAT), is a method used for detecting...
