Cell Sorting in Stem Cell Research

Cell Sorting in Stem Cell Research

Stem cells offer vast potential for new medical treatments   With the unique abilities to self-renew and rebuild functional tissues, stem cells have long been of interest to researchers as potential tools for regenerative medicine. 2006 saw a major turning point...
Challenges with Secondary Detection

Challenges with Secondary Detection

Selecting the right secondary antibodies, and optimizing them for use, is essential for reliable immunoassay data.   When developing an immunoassay, researchers typically pay close attention to primary antibodies, carefully scrutinizing the product datasheets for...
Validation of Antibodies for Flow Cytometry

Validation of Antibodies for Flow Cytometry

Application-specific validation is critical for reproducible results   Antibodies are essential tools for a broad range of research techniques. However, for data to be both accurate and reproducible, antibodies should be thoroughly validated for each of the...
Dyes for Cell Cycle and Apoptosis Analysis

Dyes for Cell Cycle and Apoptosis Analysis

Aberrant cell proliferation and cell death underlie a multitude of disease states Normal tissue homeostasis depends on a critical balance between cell proliferation and cell death. The cell cycle regulates the former, while the latter occurs via controlled...
Intravital Microscopy – Explained

Intravital Microscopy – Explained

Intravital microscopy has vast potential to reveal hidden cellular mechanisms   Intravital microscopy (IVM) is a term used to describe the direct visualization of cells and tissues within a living organism. It was first reported in the 17th century, shortly after...
Mass Cytometry – Explained

Mass Cytometry – Explained

The use of mass cytometry is growing and includes its adoption for clinical applications Mass cytometry is a variation of flow cytometry that uses antibodies labeled with metal tags rather than fluorophores. Also known as CyTOF® flow cytometry, in deference to...