Fluorescent immunoassays meet different experimental needs When immunoassays such as western blot and enzyme-linked immunosorbent assay (ELISA) were first reported, they typically produced either a radiometric or chromogenic signal. However, many common...
By measuring intracellular proteins with flow cytometry, researchers can better understand cell signaling and functional responses in conditions of health and disease Flow cytometry is widely used for identifying different cell types based on the expression of...
Choosing the right fluorophores for microscopy-based research is critical for accurate results Using fluorescent reagents for microscopy-based research offers several advantages. Not only does fluorescent detection enable multiplexing, which can be especially useful...
Common problems can often be addressed by optimizing experimental design Flow cytometry is a technique for analyzing individual cells in suspension. It works by using a stream of fluid to direct the cells in single file past an interrogation point, where one or...
Viability dyes are critical controls for proper flow analysis. The presence of dead cells can result in unwanted autofluorescence, cell aggregation, non-specific antibody binding, and a compromised capacity for RNA/DNA to proliferate in downstream applications. These...
