Articles

Flow cytometry gating can often seem like a daunting task. While there is no single solution, experienced cytometrists can recommend several tips beyond “praying for good data”. Here we define gating, explore gating methodologies, and provide some useful tips for...

This month we explore the ever-expanding selection of commercially available fluorescent antibodies and how researchers are getting better data by designing more colorful panels. Sections: Research Trend Toward More Colors Suppliers Expanding Fluorescent Catalogs...

FluoroFinder has integrated cytometer configurations for over 500 cores! (You can check for yours here). With so many researchers now using FluoroFinder to design their flow cytometry experiments, we wanted to share some of the excellent feedback we have received....

If you are working with a shared flow core facility, then you probably know how important it is to get the most out of your limited cytometer time. Aside from costing your lab time and grant money, shared cytometers are a valuable resource so booking additional time...

You may have read about a growing “reproducibility crisis”, where scientists are increasingly unable to replicate the results of their colleagues’ scientific experiments. To combat this trend, research teams are emphasizing collaboration throughout the experiment...

Analyzers & Cell Sorters Selecting the optimal cytometer for your experiment can be as important as the panel you design. We have compiled this useful list of popular cytometers for your reference. However, you should also consult your flow core manager for their...

We have compiled tips to void the 6 most common pitfalls of cytometry experiment design and analysis. Learn how to avoid failed experiments, save time and frustration and get better data! Sections: Reagent/Fluorochrome Selection Controls Sample Prep Spillover...

Note: The flow dictionary is not alphabetical, but rather grouped by the logical order of a cell as it flows through a cytometer. If you are looking for a particular term, it may be easiest to use the ctrl+f search function. Sections: Fluidics Optics & Detection...

Understanding Flow Cytometry Beads Immunobeads, or antibody-coated nanoparticles, are useful for a variety of flow cytometry-related applications. Here we outline the most commonly used bead types and explain how they can be used for cytometer calibration,...

Could a new product improve your experiment? Has that new antibody been vetted by reliable testing? Researchers have seen tremendous growth in the number of commercially available marker and dye combinations designed for flow cytometry. As new products come online,...