Optimizing Your Immunophenotyping Assay

Optimizing Your Immunophenotyping Assay

Antibody conjugate panels must be designed with care to ensure accurate results Immunophenotyping is a flow cytometry-based technique which uses fluorophore-labeled antibodies for identifying different cell types within a heterogeneous population. While some cell...
Advances in Microscopy

Advances in Microscopy

Microscopy is comprised of many innovative technologies and remains at the forefront of scientific research.   Since the invention of the microscope in the late 16th century, there has been a continual push to produce instruments with higher resolution, faster...
Guide to Fixation and Permeabilization

Guide to Fixation and Permeabilization

Fixation and permeabilization are key protocol steps for several core immunoassay techniques Immunoassay techniques such as flow cytometry, immunocytochemistry (ICC), and immunohistochemistry (IHC) often require that samples be fixed and/or permeabilized prior to...
Roundup of Spectral Flow Cytometers

Roundup of Spectral Flow Cytometers

The use of spectral flow cytometry is increasing due to the many benefits it affords. Spectral flow cytometry is a relatively new technology that was developed to push the boundaries of traditional flow cytometry. First described in 2004 by researchers at Purdue...
Near-Infrared Fluorophores For Fluorescence Imaging

Near-Infrared Fluorophores For Fluorescence Imaging

Near-infrared fluorophores offer several advantages for imaging applications. A major advantage of fluorescent detection is that it enables multiple antibodies to be combined in the same experiment. Using techniques such as immunocytochemistry (ICC) and...