Fluorescent western blot detection can offer many advantages provided protocols are carefully optimized While it was once common for researchers to use enzyme-labeled antibodies and chemiluminescent substrates to develop western blots to film, there has been a...
Spectral cytometry offers increased flexibility for fluorophore selection but researchers should still apply best practices for panel design. It has been almost 20 years since spectral cytometry was first described by the Robinson group at Purdue University...
Protocol optimization is essential for clear, reproducible results Fluorescence microscopy uses fluorophore-labeled antibodies and other fluorescent reagents to visualize targets of interest in cells and tissues. Experiments can be as simple as detecting one...
Understanding the biological density of proteins or antigens expressed by each individual cell is an imperative component of all cell-based analytical methods. The comparative change in protein expression, also called antigen density, is indicative of the...
