Structural biology is focused on mapping where proteins, lipids and other biological molecules are located in cells and tissues in space. Researchers often use immunofluorescence labelling techniques that have been around for decades to visualize biomolecules and...
Standardization may not be the most exciting topic in biomedical research, but in an era where we lament the lack of reproducibility and distribute blame to reagents, sample prep, and general technical know-how, standardization is something every researcher should...
Two-dimensional (2D) cell cultures represent a cornerstone of scientific research due to their relative simplicity and the establishment of standard techniques over time. In recent years though, 3D cell culture models have risen in popularity for the more...
Back before spectral cytometry, flow cytometers were composed of optical configurations with individual photomultiplier tubes (PMTs) or avalanche photodiodes (APDs) behind filters centered at the peak emission of different fluorophores. Many might call these...
Biologists are very interested in how proteins, lipids and other compounds are organized and interact in systems. Very few organizational details can be gained by using standard transmission-based light microscopy, even when employing phase or other contrast-enhancing...
What is Spectral Spillover? In flow cytometry, spectral spillover occurs when the emission of one fluorophore overlaps the optimal detection channel of a different fluorophore. Spillover is a concern in flow cytometry, as it can make fluorescent signatures difficult...
