Imaging Flow Cytometry

Imaging Flow Cytometry

For decades, traditional flow cytometry has been the dominant technique for analyzing single cells within large, heterogeneous populations. However, imaging flow cytometry (IFC) is rapidly becoming more mainstream due to the advantages that it can provide. We spoke...
Advancing Cytometry Technology in Oncology

Advancing Cytometry Technology in Oncology

Guest Authored By: Kelly Lundsten I often think about a concept called the pinball effect. Distilled, it is the knowledge of all of the small changes made by people whose names are remembered, or not, who optimized a process or invented a new chemistry or asked “why”...
Automation & Microfluidics in Cyclic Immunofluorescence

Automation & Microfluidics in Cyclic Immunofluorescence

Cover Image: Selected images of a melanoma specimen subjected to 20 cycles of Cyclic IF after cycles 0, 5, 15 and 20. Used without modification under Creative Commons Attribution 4.0 International License from (Lin, 2018) Figure 4G. Spatial biology aims to...
New and Emerging Spectral Flow Cytometry Instrumentation

New and Emerging Spectral Flow Cytometry Instrumentation

Spectral flow cytometry improves on traditional flow cytometry by capturing the entire emission spectrum of each fluorochrome, rather than isolating specific wavelengths. This enables more precise resolution of overlapping fluorochromes and greater flexibility in...
Autofluorescence in Flow Cytometry

Autofluorescence in Flow Cytometry

Guest Authored By: Kelly Lundsten Every living organism on earth has evolved to either harness or protect itself from the energy of the sun. Organic molecules capture energy and conduct it along a circuitry of electron pairs shared by double bonds that compose various...
Clearing Tissue for Microscopy: Part 2 – Hydrophilic Methods

Clearing Tissue for Microscopy: Part 2 – Hydrophilic Methods

Cover Image: E12.5 whole mouse embryo was immunostained with neurofilament antibody and cleared with RTF. Scale bar, 1000 μm. Used without modification under Creative Commons Attribution 4.0 International License from (Yu T. Z., 2018) Figure 4a. As explained in last...