Fluorescence-activated cell sorting (FACS) is an advanced form of flow cytometry that allows researchers to isolate specific cell types from a heterogeneous sample. This article explains the basic principles of FACS and describes some common research applications. It...
Cover Image: Imaging of the whole-brain vasculatures at sub-cellular resolution. Brains of adult Tie2-Cre;tTAflox;tetO-H2BGFP (TTH) mice were cleared using the PEGASOS method and imaged with a tiling light-sheet microscope. g An X-Z optical slice acquired at the...
When selecting reagents for flow cytometry, fluorescently-labeled antibodies are likely the first products that come to mind. However, supplementary reagents are also important and it pays to investigate what’s on offer. This article looks at some of the different...
Guest Authored By: Vera Tang, Ph.D. Interest in the study of extracellular vesicles has increased exponentially over the past two decades due to the important role they play in inter-cellular communication, as biomarkers of disease, and vehicles for drug delivery....
So many new fluorophores have been released in recent years, it can be hard to keep track of their similarities or novelty. Sometimes, a company will create a new brand name for a fluorescent chemistry that is already commonly used, which can make sifting through the...
Conjugating antibodies to other molecules is a common technique used for drug delivery, changing solubility, attaching to solid substrates (for purification or assays), and visualizing the antibody using fluorescent, magnetic or gold nanoparticles. Western blots,...
