Proper compensation is critical for accurate interpretation of your flow cytometry data. Therefore, we have compiled this “comprehensive” list of compensation tips to help improve your analysis. Sections: What is Compensation & Why Do I Need it? Best Practices for...
The “reproducibility crisis”, or the inability for researchers to replicate results or reproduce findings remains a growing concern for a wide range of scientific disciplines. This month, the National Association of Scholars published an excellent report examining the...
Posted on: Apr 3, 2018 We know you put a lot of time and effort into your flow cytometry experiments. Follow these 7 tips to ensure that your high-quality flow data is more likely to be published! Select the right cytometer Prepare your samples correctly Design a...
What is a dump channel? Simply put, a dump (also called an exclusion) channel is used to group and exclude everything that is not of interest for your study. This typically involves using one or more antibodies to stain antigens that are known not to be expressed by...
What is a dump channel? Simply put, a dump (also called an exclusion) channel is used to group and exclude everything that is not of interest for your study. This typically involves using one or more antibodies to stain antigens that are known not to be expressed by...
Cell viability dyes are critical controls for proper flow cytometry analysis. Dead cells can skew data by causing cell aggregation, contributing to cellular autofluorescence or nonspecifically binding detection antibodies. This is especially problematic when measuring...
