Image Flow Cytometry Image flow cytometry (IFC) is a technique that combines fluorescent immunostaining and microfluidics with powerful digital imaging software. Like flow cytometers, image cytometers analyze heterogeneous cell populations across multiple parameters...
The replication crisis, or data irreproducibility crisis, is now a well-established problem across all scientific disciplines. This inability for researchers to replicate published experimental results exposes data integrity issues and risks potentially faulty...
Flow Cytometer/Cell Sorter Instrument Types Flow cytometers and cell sorters are two powerful tools for the accurate and efficient characterization of cells. Both instrument types measure forward scatter (FSC), side scatter (SSC) and multiple fluorescent parameters...
Advances in flow cytometry instrumentation, technology and chemistries have enabled researchers to expand their experimental parameters rapidly. Experiments that were once limited to the detection of three to four parameters have increased to detect up to 40 compounds...
Fluorescent proteins are an important part of any molecular biologist’s toolkit. Recombinant DNA techniques allow researchers to incorporate a fluorescent protein into an engineered plasmid and track its expression over time with flow cytometry or, more commonly,...
The enzyme-linked immunosorbent assay (ELISA) offers a simple and effective method for rapid biomarker detection. The main benefits of ELISA include good specificity, low cost, and straightforward readout. However, the limited sensitivity of standard colorimetric...
