One of the most common complaints when using fluorescently-labeled antibodies for staining biological samples is that the specific signal is too low. Another is that the background is too high, obscuring detection of the target. We spoke with Eric Torres, Ph.D.,...
Recombinant antibodies have become indispensable tools for flow cytometry due to the advantages that they offer over traditional polyclonal and monoclonal antibodies. We spoke with Mathilde de Jong, Ph.D., Product Manager for Flow Antibodies at Miltenyi Biotec, and...
DNA-PAINT (DNA points accumulation for imaging in nanoscale topography) is a super-resolution microscopy technique that exploits the transient binding of fluorescently labeled DNA probes. It has been widely adopted for scientific research owing to its accessibility,...
Flow cytometry requires more controls than most other immunoassay techniques because it must account for more potential sources of variation. In addition to experimental controls, which are used to verify the specificity of any staining and ensure assay consistency,...
It has been almost half a century since Georges Köhler and Cesar Milstein first described hybridoma technology, which is still widely used for producing monoclonal antibodies today. Over the years, the methodology has evolved and alternative approaches have been...
When using established methods for immunofluorescent staining of tissue sections, the need to avoid spectral overlap limits the number of markers that can be detected. This can mean that important information is missed, which is of particular significance when working...
