Historically, fluorescence microscopy was limited by the number of parameters that could be imaged on a single sample due the spectral overlap of the fluorophores available at the time. There was a prevailing dogma that only one fluorophore could be assigned to each...
Immunohistochemistry (IHC) is an antibody-based technique for detecting specific analytes in tissue sections. Because the tissue architecture is preserved, it is possible to study the distribution and relative abundance of targets of interest, both in the context of...
Whether you are using a traditional cytometer that employs compensation and does not exceed 18 colors or a spectral cytometer that employs an unmixing algorithm and has the potential, currently, to analyze 50 color panels, the basic tenets of flow cytometry panel...
