Pathologists and life science researchers are increasingly performing multiplexed assays on formalin-fixed, paraffin-embedded samples. This allows for the collection of more data from a single tissue specimen, far beyond the typical single-color immunohistochemistry...
Compensation is the process of correcting for spillover when one fluorophore is detected in multiple channels. It is required for most experiments of four or more colors to identify the correct signal that should be measured in each channel. This quick list can help...
When designing a flow cytometry experiment, it is important to consider the relative brightness of each fluorescent label on your specific instrument. Generally, it is best to assign brighter fluorochromes to weakly expressed markers, and dimmer labels to strongly...
Flow cytometry can be performed directly using a fluorescently labeled primary antibody, or indirectly using an unconjugated primary antibody with a labeled secondary. Most researchers prefer direct detection due to its simplicity and ease of use. However, certain...
The inability to resolve signals with overlapping color spectra has traditionally limited fluorescence microscopy to a “color-barrier” of 5 colors. However innovative new developments in microscopy techniques and analysis software are allowing researchers to resolve...
