Flow cytometry cell cycle analysis typically involves using a DNA binding dye to determine each cell’s total DNA content. Sorting cells by cell cycle phase is then possible, as the total DNA content fluctuates as cells pass through G0/G1, S, and G2/M phases. Cells in...
The first step to any successful flow cytometry data analysis is proper gating. For a complete overview of gating terminology, types and strategies, check out our previous Gating Newsletter. Here we outline 6 questions that cytometry researchers should ask themselves...
Flow cytometry remains the most powerful tool for studying apoptosis in cell populations. However, many researchers may not be aware of the variety of options available for apoptosis markers. Here we explore the use of flow cytometry in apoptosis research and examine...
Cytometer technology is continually improving, with more lasers, detectors, and channels to allow the measurement of more parameters. But researchers are too often reluctant to expand the scope of their experiments for fear of increasing design complexity. This month...
This month we explain why antigen density is important to flow cytometry studies, describe how it is measured, and list some useful references for known antigen expression densities… 1. Why Antigen Density Matters 2. Methods for Determining Antigen Density 3....
