OMIPs: Stop Reinventing the Wheel
Optimized Multicolor Immunofluorescence Panels (OMIPs) refer to a set of validated reagents that can be used together to characterize a specific cell state or response via flow cytometry or fluorescence microscopy. Intended to alleviate development time for researchers while acknowledging the developers’ work, OMIPs serve as a useful starting point for researchers designing new experiments on similar sample types.
How does an OMIP get approved?
The three criteria for accepting new OMIPs are:
- Novelty – the OMIP must represent a unique combination of 5 or more reagents designed to address a specific question that are not yet adequately addressed.
- Optimization – the OMIP must provide documentation for why particular reagents were selected, and how the final panel of reagents and fluorochromes was devised.
- Utility – the panel should be useful to a range of researchers.
The submission cover letter must address all three of these points in detail.
Adapt a Published OMIP using FluoroFinder
To date, Cytometry Part A has published 44 total approved OMIPs. These panels mainly focus on blood and immunology research on T-cell or leukocyte populations from human, mouse, or monkey samples. Researchers designing similar experiments are encouraged to review the OMIP list and use these pre-validated panels as a resource for their own panel development.
“When we first introduced the OMIP publication format we had also envisioned a searchable online tool that would link to relevant OMIPs according to a selected cell type, markers, or species. FluoroFinder is working to integrate OMIPs into its online panel building platform, which promises to make alteration of previously optimized panels even easier”, said flow cytometry consultant and original OMIP developer Yolanda Mahnke, PhD. “This will not eliminate the need to thoroughly test any panel before its application in your studies, but it will shorten the process.”
Publish Your Panel as the Next OMIP
Have you developed a multicolor panel that you feel would be beneficial to share with others studying in your field? Submit your OMIP proposal to Cytometry Part A for recognition in the publication. Follow the guidelines laid out in the introductory OMIP series article.