All About FACS

All About FACS

Fluorescence-activated cell sorting (FACS) is an advanced form of flow cytometry that allows researchers to isolate specific cell types from a heterogeneous sample. This article explains the basic principles of FACS and describes some common research applications. It...
Clearing Tissue for Microscopy: Part 1 – Dehydration Protocols

Clearing Tissue for Microscopy: Part 1 – Dehydration Protocols

Cover Image: Imaging of the whole-brain vasculatures at sub-cellular resolution. Brains of adult Tie2-Cre;tTAflox;tetO-H2BGFP (TTH) mice were cleared using the PEGASOS method and imaged with a tiling light-sheet microscope. g An X-Z optical slice acquired at the...
Flow Cytometry of EVs and Other Small Things

Flow Cytometry of EVs and Other Small Things

Guest Authored By: Vera Tang, Ph.D. Interest in the study of extracellular vesicles has increased exponentially over the past two decades due to the important role they play in inter-cellular communication, as biomarkers of disease, and vehicles for drug delivery....