Webinars
On-Demand Webinars
This collection of webinars speaks to experimental design across industries that utilize tools that FluoroFinder offers on our platform. We regularly update these webinars as an invaluable resource for our community of users.
Sort with Confidence – Ease of Use, Purity, Viability, and Cell Function across Multiple Applications
Speaker: Larry Wong, PhD, Senior Application Scientist, Sony Biotechnology
In this webinar, learn how the MA900 Cell Sorter can be used for multiple applications. We’ll discuss how to optimize system setup for gentle sorting of multicolor panels of immune and stem cells, and single-cell sorting of cancer cell lines.
Evolution of High Parameter Spectral Solutions – Integrating ID7000™ Spectral Analyzer and FP7000 Spectral Sorter Workflows
Speaker: Andy Draghi, PhD, Field Applications Scientist, Sony Biotechnology
In this webinar, we describe the design of 20+ color panels, complete detailed analysis of results using the ID7000™ Spectral Cell Analyzer, and how to sort subsets of interest using the FP7000 Cell Sorter.
Preventing Is Better Than Fixing – How To Optimize Controls And Generate A Correct Spectral Unmixing Matrix
Speaker: Dr. Mirko Corselli, Associate Director, Market Development, BD Biosciences
In this webinar, we will comprehensively review all the main factors that may contribute to spectral unmixing errors and describe the control optimization process.
Is it Fluorophores or Fluorochromes?
Speaker: Kelly Lundsten, VP of Strategic Product Innovation, FluoroFinder
This webinar is an introduction to help you understand fluorophores in the context of cytometry applications.
The Use of MultiDots in Fluorescence-Linked Immunosorbent Assays (FLISAs)
Speaker: Dr. Mythreyi Unni, Principal Research Scientist, Core Quantum Technologies, Inc.
In this webinar, we discuss a step by step protocol implementing the use of MultiDots in a fluorescence-linked immunosorbent assay (FLISA) platform.
The Balancing Act Between Number Of Colors And Data Quality: Lessons Learned From Human Memory T Cells
Speaker: Maria C Jaimes, MD, VP Scientific Commercialization, Cytek Biosciences, Inc.
In this webinar, we discuss the importance of balancing quality and quantity when optimizing robust multiparametric assays, empowered by Cytek’s Full Spectrum Profiling™ (FSP™) technology.
Tips and Tricks for Conventional and Spectral Flow Cytometry: How to Optimize Workflows to Maximize Biological Resolution
Speaker: Seddon Y. Thomas, Ph.D., Staff Scientist, R&D, Protein & Cell Analysis, Thermo Fisher Scientific
In this webinar, we explore optimized workflows and strategies to address common challenges with the transition from conventional to spectral flow cytometry.
Novel Fluorescent and Magnetic Reagents for Life Science Research: The MagDot
Speaker: Kristie Krug, CEO of Core Quantum Technologies
This webinar focuses on the use of MagDots in magnetic cell separation, flow cytometry analysis, and rare protein expression in Yeast Display Libraries. We also discuss protocols, troubleshooting, and types of magnets used in the separations.
Utilizing the ID7000™ Spectral Cell Analyzer to Profile the Human Immune System: Unraveling Immune Profiles and Diseases
Speaker: Laurien Waaijer, PhD Student, Laboratory of Medical Immunology at Radboud University Medical Center
In this webinar, we discuss the use of the ID7000 system in designing a 42-parameter immunoprofiling panel.
Targeted Tumor Destruction: High-Throughput Assessment of ADC Cytotoxicity using Flow Cytometry
Speaker: Kirsty McBain, Scientist, Analytics, Sartorius
In this webinar, we demonstrate the application of high-throughput flow cytometry for evaluating the efficacy of ADCs that target the HER2 protein on cancer cells.
Optimizing Controls for Flow Cytometry
Speaker: Amay Dankar, M.S., Associate Director of Field Applications, Slingshot Biosciences
In this educational webinar, Amay discusses common controls that users need to run their best experiments and the prerequisites for running optimized panels.
Novel Fluorescent and Magnetic Reagents for Life Science Research
Speaker: Kristie Krug, CEO of Core Quantum Technologies
In this webinar, Kristie discusses the development and application of Core Quantum Technology’s highly stable fluorescent reagents with narrow emissions and greater tunability, as well as their unique magnetic and fluorescent reagent that allows for magnetic separation and immediate fluorescent labeling.
An Inflammatory State Remodels the Immune Microenvironment and Improves Risk Stratification in Acute Myeloid Leukemia
Speaker: Audrey Lasry, PhD Department of Pathology, NYU Langone.
In this webinar, we discuss the framework for classifying AML patients based on their immune microenvironment and the use of CITE-Seq to characterize inflammatory responses and the immune microenvironment in AML.
Deriving an Advanced Flow Cytometry Framework for Immunological Data
Speaker: Nina Senutovitch PhD, Senior Scientist, Sartorius.
In this webinar, we discuss how to harness adaptive immune systems to leverage adoptive cell therapies and use advanced flow cytometry to overcome some of the challenges with ACT.
Debunking Antibody Titration Myths
Speaker: Patrick Duncker, Technical Lead, Cytek Biosciences.
In this webinar, we discuss the best practices for antibody titrations, metrics used to evaluate optimal titer, and the impact of flow cytometer setup and configuration on antibody titer.
What’s the Deal with Your Compensation and Unmixing?
Speaker: Andreas (Jack) Panopoulos, PhD, Slingshot Biosciences, Inc.
In this webinar, we discuss the powerful rules governing proper compensation controls and employment of the spillover spreading matrix to reduce errors and avoid loss of resolution, respectively.
A Serum-Free, Chemically-Defined Workflow for T Cell Therapy
Speaker: Zayda Piedra, PhD, Product Development Scientist at BioLegend
In this webinar, we will go over current challenges in the cell therapy industry and what we can do better, BioLegend’s emerging solutions for T cell therapy – closing the gaps, one at a time, and the importance of bioprocessing reagents manufactured with consistent, high-quality well-defined components.
Highly Multiplexed Imaging of Diverse Tissues Using IBEX
Speaker: Andrea Radtke, Ph.D. and Dr. Ziv Yaniv, Ph.D.
In this webinar, we will go over methods to study normal and malignant lymph nodes and topics related to multiplexed tissue imaging, namely: optimal tissue processing, working with fixed frozen and FFPE specimens, overcoming autofluorescence, identifying appropriate reagents, validating antibodies, and creating organ mapping antibody panels (OMAPs).
MA900 Cell Sorter: Increasing Application Capabilities in Multi-User Laboratories
Speaker: Anthony Carcio, Field Application Scientist, Sony Biotechnology
This webinar will provide insights to researchers who are new to sorting or who operate shared flow facilities on how the Sony MA900 can support varied user needs and guided workflows for sorting. Learn how an automated and streamlined sort setup can increase the capabilities within a multi-user lab, review how the MA900 enables seamless setup and exchange of different chips without instrument downtime between users, and understand how the MA900 can be maintained in multi-user labs for successful, high-throughput, and gentle sorting of various cells.
Evaluation of Large Antibody Panels in Single-Cell Genomic Immunophenotyping of Fresh and Frozen Human Leukocytes
Speaker: Dr. Jasmine Ying-Jiun Chen
Dr. Chen will share Abiosciences’ effort in evaluating BioLegend’s TotalSeq™-C Human Universal Cocktail using the 10x Genomics single-cell platform on fresh and frozen PBMCs. This includes bioinformatics pipelines to compare the quality control metrics, isotype matched control performance, and cell subtype analyses. Advanced visualization and analysis tools were developed to interrogate the data in a flow cytometry paradigm, including complex gating strategies to identify rare cell subtypes. The pilot study is being expanded to larger scale clinical settings and may help characterize important cell populations that are associated with disease status, pharmacodynamics, and therapeutic response.
Cell Painting – A Practical Approach
Speaker: Joe Trask, North America HCS Product Line Leader, Cellular Imaging and Analysis, PerkinElmer, and Alexander Schreiner Team Leader, Biological Applications Team, PerkinElmer, Hamburg, Germany
Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology. Specific cellular compartments and organelles are simultaneously stained with six fluorescent probes, followed by imaging and analysis to extract hundreds to thousands of features to phenotypically profile the cells and gain a deeper understanding of the effects of chemical, drug, or genetic perturbagens.
High-Dimensional High-Throughput Rare Event Immunophenotyping on the ID7000™ Spectral Cell Analyzer
Speaker: Jeff Clapper, Senior Field Applications Scientist Sony Biotechnology Inc.
The ID7000™ spectral cell analyzer is ideal for high-dimensional flow cytometry. More lasers and many more detectors mean more fluorochromes can be resolved in combination.
Software Workflows and Tools that Enable High Parameter Flow Cytometry using the ID7000™ Spectral Cell Analyzer
Speaker: Vendula Sinkorova, Senior Field Application Scientist, Sony Biotechnology Inc.
In this webinar from Sony, we will review the workflows to highlight the ease of operating the ID7000™ spectral cell analyzer to obtain high-parameter, high-quality data.
Measuring T Cell Exhaustion: A Multiplexed Approach Utilizing Advanced Flow Cytometry
Speaker: Mark Carter, Manager of Assay Development. Product Development Cell Analytics, Sartorius
Understanding immune system dynamics and their role in health and disease, as well as developing novel therapies, relies on the use of powerful analytical screening tools to probe cellular functions. Characterizing immune cell function such as the induction and persistence of T cell exhaustion is important for furthering therapeutic interventions.
AccuCheck ERF Reference Particles: Easy to Use Flow Cytometry Intensity Reference with Accurate NIST-Assigned Values
Adam W. York, Ph.D. Sr. Staff Scientist in Chemistry R&D at Thermo Fisher Scientific
A study initiated by the International Society for the Advancement of Flow Cytometry (ISAC) found that microspheres with assigned MESF (molecules of equivalent soluble fluorophore) values were not always accurate and differed considerably from one manufacturer to another. To address these inconsistencies, the ERF (equivalent reference fluorophore) intensity unit was introduced by the National Institute of Standards and Technology (NIST).
SARS-CoV-2 Infection as a Potential Trigger for Diabetes: The Forest and The Trees
Speaker: Dirk Homann, MD, PhD, MA, Professor of Medicine at Icahn School of Medicine at Mount Sinai
The relationship between SARS-CoV-2 infection and diabetes has been proposed to exhibit a “bidirectional dynamic”, where pre-existing diabetes may exacerbate the course of COVID-19, and SARS-CoV-2 infection, in turn, may precipitate diabetes.
Streamlining and Democratizing Functional Testing by Flow Cytometry
Speaker: Jean-Marc Busnel, Ph.D. Senior Staff Research Scientist, Beckman Coulter Streamlining and Democratizing Functional Testing by Flow CytometryWebinar Topics Include: 1) Allergy testing with streamlined basophil activation testing2) Flow cytometry based...
MACSQuant® Tyto®: Plug & play cell sorting in a fully closed cartridge system
Speaker: Dr. Felix Eppler, Ph.D. Junior Product Manager, Miltenyi Biotec In this webinar, you will learn about the microchip-based sorting technology of the MACSQuant® Tyto® Cell Sorter. Find out how cell sorting within a closed cartridge system eliminates the risk of...
Conventional and Spectral Flow Cytometry Panel Building and Data Analysis with FCS Express
Speaker: Sean Burke – De Novo Software and Jeff Jensen – FluoroFinder De Novo Software and FluoroFinder are collaborating to help bridge the gap from experiment design to data analysis for both conventional and spectral flow cytometry. The one hour webinar will...
5 Most Useful Flow Cytometry Panel Design Features on FluoroFinder
Speaker: Jeff Jensen, Flow Cytometry Lead & Head of Sales, FluoroFinder One of the biggest challenges of multi-color flow cytometry is selecting the right combination of fluorochromes and antibody conjugates that fit your cytometer so that the quality of data is...