OMIPS
OMIP Panels Based on Cell Population Being Profiled
Optimized Multicolor Immunofluorescence Panels (OMIPs) are peer-reviewed panels designed for fluorescent assays. FluoroFinder can guide you through the process of utilizing an OMIP as part of your panel design process.
CD4 OR CD8 T CELLS
OMIP-001: Quality and phenotype of Ag-responsive human T-cells
OMIP-002: Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes
OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells
OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells
OMIP-009: Characterization of antigen-specific human T-cells
OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice
OMIP-013: differentiation of human T-cells
OMIP-014: validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining
OMIP-015: human regulatory and activated T-cells without intracellular staining
OMIP-016: Characterization of antigen-responsive macaque and human T-cells
OMIP-017: human CD4(+) helper T-cell subsets including follicular helper cells
OMIP-018: chemokine receptor expression on human T helper cells
OMIP-020: Phenotypic characterization of human γδ T-cells by multicolor flow cytometry
OMIP-021: Simultaneous quantification of human conventional and innate-like T-cell subsets
OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory
OMIP-023: 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes
OMIP-024: Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples
OMIP-025: evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining
OMIP-030: Characterization of human T cell subsets via surface markers
OMIP-031: Immunologic checkpoint expression on murine effector and memory T-cell subsets
OMIP-033: A comprehensive single step staining protocol for human T- and B-cell subsets
OMIP-034: Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies
OMIP-036: Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets
OMIP-042: 21-color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood
OMIP-050: A 28-color/30-parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules
OMIP-052: An 18-Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques
OMIP-056: Evaluation of Human Conventional T Cells, Donor-Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining
OMIP-058: 30-Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells
OMIP-060: 30-Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T Cells
OMIP‐062: A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples
OMIP-063: 28-Color Flow Cytometry Panel for Broad Human Immunophenotyping
OMIP-067: 28-Color Flow Cytometry Panel to Evaluate Human T-Cell Phenotype and Function
OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood
OMIP 071: A 31-Parameter Flow Cytometry Panel for In-Depth Immunophenotyping of Human T-Cell Subsets Using Surface Markers
OMIP 072: A 15-color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemia
OMIP 073: Analysis of human thymocyte development with a 14-color flow cytometry panel
OMIP 075: A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primates
OMIP 076: High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsets
OMIP 078: A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells
B CELLS
OMIP‐003: Phenotypic analysis of human memory B cells
OMIP‐026: Phenotypic analysis of B and plasma cells in rhesus macaques
OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland
OMIP‐033: A comprehensive single step staining protocol for human T‐ and B‐cell subsets
OMIP‐043: Identification of human antibody secreting cell subsets
OMIP‐047: High‐Dimensional phenotypic characterization of B cells
OMIP‐051: 28‐color flow cytometry panel to characterize B cells and myeloid cells
OMIP‐068: High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic Infection
OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood
OMIP-074: Phenotypic analysis of IgG and IgA subclasses on human B cells
OMIP 077: Definition of all principal human leukocyte populations using a broadly applicable 14-color panel
NK CELLS
OMIP‐007: Phenotypic analysis of human natural killer cells
OMIP-024: Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples
OMIP‐027: Functional analysis of human natural killer cells
OMIP‐028: Activation panel for Rhesus macaque NK cell subsets
OMIPS-029: Human NK-cell phenotypization
OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland
OMIP‐035: Functional analysis of natural killer cell subsets in macaques
OMIP‐037: 16‐color panel to measure inhibitory receptor signatures from multiple human immune cell subsets
OMIP‐039: Detection and analysis of human adaptive NKG2C+ natural killer cells
OMIP‐064: A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells
OMIP‐070: NKp46‐Based 27‐Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues
DENDRITIC CELLS (DCS)
OMIP‐038: Innate immune assessment with a 14 color flow cytometry panel
OMIP‐044: 28‐color immunophenotyping of the human dendritic cell compartment
OMIP‐051: 28‐color flow cytometry panel to characterize B cells and myeloid cells
OMIP‐061: 20‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting Cells
OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood
REGULATORY T CELL (TREG)
OMIP‐004: In‐depth characterization of human T regulatory cells
OMIP‐006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry
OMIP‐015: Human regulatory and activated T‐cells without intracellular staining
OMIP-048: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry
OMIP‐053: Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg Subsets
INNATE LYMPHOID CELLS (ILC)
OMIP‐055: Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood
OMIP‐066: Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood
OMIP‐062: A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples
OMIP‐064: A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells
OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood
OTHER HEMATOPOIETIC/IMMUNE CELLS
OMIP‐010: A new 10‐color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples
OMIP‐019: Quantification of human γδT‐cells, iNKT‐cells, and hematopoietic precursors
OMIP-046: Characterization of invariant T cell subset activation in humans
OMIP‐049: Analysis of Human Myelopoiesis and Myeloid Neoplasms
OMIP‐059: Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining Panel
OMIP-065: Dog Immunophenotyping and T-Cell Activity Evaluation with a 14-Color Panel
TISSUE DERIVED CELLS
OMIP‐040: Optimized gating of human prostate cellular subpopulations
OMIP‐041: Optimized multicolor immunofluorescence panel rat microglial staining protocol
OMIP‐045: Characterizing human head and neck tumors and cancer cell lines with mass cytometry
OMIP-054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry
OMIP-057: Mouse γδ T-Cell Development Characterized by a 14 Color Flow Cytometry Panel
References and Collaborators:
1. Wei Wang, MD. The Columbia Center for Translational Immunology (CCTI) at Columbia University Irving Medical Center.” Cytometry Part A