Cytometers

Analyzers, and Cell Sorters

Selecting the best flow cytometers, analyzers, and proper cell sorting devices can be as important as the panel design. This compiled list of popular cytometers is meant to serve as a helpful reference and resource for experimentation. We recommend also consulting your flow core manager for their recommendation.

Analyzers

best flow cytometers, Acuri C6 Plus

Accuri™ C6 Plus – BD Biosciences

The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. The BD Accuri C6 Plus supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications.

Lasers: 2 (red, blue)
Detectors: 2 light scatter, 4 fluorescence
Colors: Up to 4
Footprint: 11″ x 14.75″ x 16.5″
Weight: 30 lbs

Advanteon – ACEA Biosciences, best flow cytometers

Advanteon – ACEA Biosciences

The NovoCyte Advanteon™ is a simple and flexible instrument that you can start small with and easily upgrade. It can be configured as a one, two, or three lasers set up with up to 21 fluorescent channels plus forward and side scatter. Additionally, the Advanteon is configurable with the NovoSampler Q (pictured here), an automatic sample loading system capable of rapid and high-throughput reading and compatible with a variety of sample formats such as a 40 tube rack or 24/48/96/384 well plates.

Lasers: From one to three lasers (405 violet, 488 blue, 561 green-yellow, or 640 red)
Detectors: Up to 23 (21 fluorescent and two scatter)
Colors: Up to 21
Footprint: 24.4″x 18.1″ x 18.8″
Weight: 86 lbs

Attune™ NxT, best flow cytometers

Attune™ NxT – ThermoFisher Scientific (Invitrogen)

The Invitrogen Attune NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer with up to 4 lasers and 16 parameters of detection. The Attune NxT Flow Cytometer was developed with the goal of removing barriers associated with flow cytometry and enabling new science. This includes bringing the power of flow cytometry to the analysis of more sample types, including cancer cells. The addition of the Invitrogen Attune Autosampler helps enable significantly faster high throughput multiparametric screening.

Lasers: Up to 4 (Violet 405, Blue 488, Yellow 561, Green 532, Red 637)
Detectors: 16
Colors: 14
Footprint: 16” x 23” x 17”
Weight: 64 lbs
Links: thermofisher.com/attune
Documents: attune-nxt-flow-cytometer-spec-sheet

Aquios™ , best flow cytometers

Aquios™ – Beckman Coulter

The Aquios CL Flow Cytometry System streamlines operations by incorporating automated loading, sample preparation, reagent management, and barcode scanning as well as data analysis and bidirectional LIS connectivity in one compact platform. This analyzer was developed to remove the bottleneck that usually occurs with sample preparation and data management in routine flow cytometry. With one company platform, it semi-automates the process and increases your laboratory’s productivity.

Lasers: 488 solid-state diode
Detectors: 8 total (5 fluorescence, forward scatter, side scatter, and electronic volume)
Footprint: 32” x 22” x 22”

Aurora - Cytek® Biosciences, best flow cytometers

Aurora - Cytek® Biosciences

The Cytek Aurora is the company’s flagship spectral analyzer. It is highly configurable and incorporates cutting edge technology found in no other instruments to deliver accurate results while analyzing complex multicolor experiments. Cytek’s proprietary technology of high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor detector arrays allow the instrument to separate and collect light very efficiently from 365-829 nm. The Cytek system allows users to save and reuse reference control data from single-stained and unstained samples so that reference controls are not necessarily needed with each experiment. Teamed with Cytek’s Automatic Micro-Sampling System (AMS) and SpectroFlo Software, this instrument is a proven and powerful tool for a wide variety of applications.

Lasers: Three laser (405, 488, 640) base model, can upgrade to four or five lasers (355, 561)
Detectors: Up to 67 (64 fluorescent plus three scattering) channels for detection over the full emission spectra
Colors: Over 30 colors
Footprint: 21.3″ x 20.5″ x 20.5″
Weight: 176 lbs

BriCyte E6 - Mindray, best flow cytometers

BriCyte E6 - Mindray

The BriCyte E6 cytometer system is equipped with dual-laser-based optics. A red diode laser and a blue diode laser are used for exciting 4 to 6 colors of fluorescence lights. The optics are designed with state-of-the-art technology to ensure higher sensitivity for better identification of cellular populations.

Lasers: Blue solid-state diode (488nm, 43mW), Red solid-state diode (638nm, 55mW)
Detectors: FSC, SSC, FITC, PE, PerCP/PerCP-Cy5 or ECD/PI, APC, PE-Cy7, APC-Cy7
Colors: 4-6

 

CyAn™ ADP – Beckman Coulter, best flow cytometers

CyAn™ ADP – Beckman Coulter

The CyAn ADP High-Performance Flow Cytometer is a state-of-the-art flow cytometer that utilizes multiple laser excitation sources to analyze biological cells, beads, or other microscopic particles. The CyAn ADP has state-of-the-art optics, utilizes high-performance digitization with pulse processing speed up to 70,000 events per second complementing a high acquisition and analysis capability of up to 100,000,000 events.

Available in 7 and 9 color configurations.

Lasers: 2 (Violet 405, Red 642) or 3 (add Blue 488)
Detectors:  Up to 9 or 11 parameters
Colors: Up to 7 or 9 color configurations
Footprint: 12” x 18” x 16”
Weight: 88 lbs

CyFlow® Cube - Sysmex, best flow cytometers

CyFlow® Cube - Sysmex

The CyFlow® Cube series, CyFlow Cube 6 and the CyFlow Cube 8 instruments are fully integrated, compact, robust FCM solutions that will meet your daily research laboratory needs. These innovative, stand-alone flow cytometers offer all you need to precisely acquire cells or particles from samples in a powerful single device.

CyFlow® Cube 6
Lasers: 
2 (Blue 488, Red 638)
Detectors:  Up to 6 parameters
Colors: Up to 4 colors
Footprint: 15” x 11” x 11.5”

CyFlow® Cube 8
Lasers:  3 (UV 375, Violet 405, Blue 488, Green 532, Yellow 561, Orange 594, Red 638)
High power UV-LED: 365nm
Detectors:  Up to 8 parameters
Colors: Up to 6 colors
Footprint: 19.5” x 18.5” x 14.5”

Also available as CyFlow® Cube 8 Sorter
Detectors: Up to 5 optical parameters
Colors: Up to 3 colors

CytoFLEX - Beckman Coulter, best flow cytometers

CytoFLEX - Beckman Coulter

Providing quality and performance at any configuration, the CytoFLEX system provides powerful sensitivity and resolution for the simple to the most challenging applications. CytoFLEX delivers and surpasses capabilities expected in top tier analyzers, with excellent performance and nanoparticle resolution.

Available in 21 flexible configurations.

CytoFlex
Lasers:  3 (Violet 405, Blue 488, Red 638)
Colors: Up to 13 colors

CytoFlex S
Lasers:  4 (Available in four 4-laser configurations)
Colors: Up to 13 colors

CytoFlex LX
Lasers:  6 (Blue, Red, Violet, Yellow Green, Near UV, Infrared)
Colors: Up to 21 colors

Footprint: 16” x 16” x 13”

 

FACSArray™ – BD Biosciences, best flow cytometers

FACSArray™ – BD Biosciences

The BD FACSArray™ bioanalyzer is for fast and sensitive high-content measurements of cells and proteins in cell biology, immunology, and proteomics. This compact flow cytometer rapidly detects and quantifies concentrations of secreted proteins, proteins in cell lysates, and cell-associated proteins using small sample volumes directly from a 96-well microtiter plate through multiplexed BD™ Cytometric Bead Arrays (CBAs). Furthermore, it supports your search for answers in cellular analysis of apoptosis, cytokine and chemokine profiling, and phosphorylation of key signal transduction proteins. The bioanalyzer can also be used to assess hybridomas, cell viability, proliferation, and immunotoxicology.

FACSymphony is highly customizable.

Lasers:  2 (green 532 & red 635)
Channels: Up to 6 (four fluorescence and two scatter)
Footprint: 25.3″ x 22.1″ x 19.6″
Weight: 85 lbs.

FACSCanto™ II – BD Biosciences, best flow cytometers

FACSCanto™ II – BD Biosciences

The BD FACSCanto™ II system features many innovations. At the heart of the cytometer, for example, the fluidics system features a fixed-alignment flow cell to minimize startup time and improve reproducibility. The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate.

Lasers: Available in 2 (Blue 488, Red 633) and 3 (add Violet 405)
Detectors:  Up to 10 parameters
Colors: Available in 6 and 8 color options
Footprint: 24” x 36” x 25”

FACSCelesta™ - BD Biosciences, best flow cytometers

FACSCelesta™ - BD Biosciences

The BD FACSCelesta™ flow cytometer is designed to make multicolor flow cytometry more accessible and allow researchers to benefit from new innovations in instrument and reagent technology. Parallel advances in optical and reagent technology are pairing multilaser, multidetector instruments with bright new dyes, enabling increasingly deep and powerful insights in cell analysis.

 

Lasers: 2-3 lasers
violet 405 and blue 488 (plus red 640, ultraviolet 355, or yellow-green 561)
Detectors:  Up to 14 parameters
Colors: Up to 10 colors
Footprint: 23″ x 23″ x 24″
Weight: 110 lbs

FACScan™ – BD Biosciences, best flow cytometers

FACScan™ – BD Biosciences

The FACScan™ system is an automated flow cytometer. It analyzes cells as they pass through a focused laser beam one at a time in a moving fluid stream. A BD FACScan flow cytometer can be used for routine research applications, immunophenotyping, and DNA cell-cycle analysis.

Lasers:  488, 530, 575 or 650
Channels: 5 acquisition channels

 FACSLyric™ – BD Biosciences, best flow cytometers

FACSLyric™ – BD Biosciences

The BD FACSLyric is fast, simple to use, and automation friendly. The instrument comes with up to three lasers (blue, red, violet) and comes with 4 to 12 fluorescent channels, amounting to 14 measurable parameters including scattering channels. Users can start small and upgrade the instrument in the future. Additionally, when equipped with the BD FACS Universal Loader, there are 21 different loading options for this instrument from plates to tubes. This platform is available in a research grade instrument as well as a clinical grade instrument for clinincal cell analysis.

 Lasers: From one to three lasers (405 violet, 488 blue, or 640 red)
Detectors: From 4 to 12 colors plus two scattering channels for 14 total parameters
Colors: Up to 12
Footprint: 24.9″ x 22.8″ x 22.8″
Weight: 123.5 lbs (+29 lbs for loader)

 

FACSymphony™ – BD Biosciences, best flow cytometers

FACSymphony™ – BD Biosciences

The BD FACSymphony™ system is a novel cell analyzer that leverages the inherent benefits of flow cytometry and enables the simultaneous measurement of up to 50 different characteristics of a single cell. This high parameter flow cytometer is a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations.

FACSymphony is highly customizable

Lasers:  Up to 10 (choose from 25 wavelength options)
Channels: Up to 50

FACSVerse™ – BD Biosciences, best flow cytometers

FACSVerse™ – BD Biosciences

The BD FACSVerse™ flow cytometer is designed to support cell analysis for research applications using up to 10 parameters. Hardware innovations ensure the reliability of results and signal resolution. BD FACSuite™ software delivers a seamless workflow from system setup, through data acquisition, analysis, and shutdown.

Lasers: Available in 1 (blue 488), 2 (blue 488, red 640), and 3 laser (violet 405, blue 488, red 640) configurations
Detectors:  Up to 10 parameters
Colors: Available for 4, 6 and 8 colors analysis
Footprint: 33.5″ x 22.8″ x 22.8″
Weight: 110 lbs

FACSVia™ – BD Biosciences, best flow cytometers

FACSVia™ – BD Biosciences

With BD FACSVia™ system, you get a powerful, easy-to-use benchtop system designed to ensure that your laboratory runs smoothly and efficiently. The BD FACSVia system provides accuracy, repeatability, and ease-of-use–along with low cost of operation–from a system that combines space-saving innovations in optics, fluidics, electronics, and miniaturization.

Lasers: 2 (blue 488, red 640)
Detectors:  6 ( two light scatter detectors, and four fluorescence detectors)
Colors: Available for 4, 6 and 8 colors analysis
Footprint: 11″ x 14.75″ x 16.5″
Weight: 30 lbs

 

FACSCount™ - BD Biosciences

FACSCount™ - BD Biosciences

The BD FACSCount™ system provides absolute and percentage results of CD4 T lymphocytes to stage progression of HIV/AIDS, to guide treatment decisions for HIV-infected persons, and to evaluate effectiveness of therapy in a wide range of laboratory settings. A proven, turnkey solution, the BD FACSCount system has been recognized for its simplicity of operation, robust performance, and reliable results for over a decade. Today clinicians in programs throughout Africa, Asia, Eastern Europe, and Latin America rely on the system for cost-effective, simple, and dependable CD4 testing.

For In Vitro Diagnostic Use
Lasers: 1
Footprint: 17” x 15” x 22”
Weight: 57 lbs

FC 500 – Beckman Coulter, best flow cytometers

FC 500 – Beckman Coulter

The Cytomics FC 500 Series is a sophisticated product family equipped with the most advanced technologies available. The FC 500 Series delivers flexible testing, powerful software and a user-friendly interface – features that help scientists optimize their overall workflow efficiency while maintaining true cost effectiveness. Plus, each FC 500 Series system is backed by Beckman Coulter’s unsurpassed reputation for quality and service.

Lasers: 2 (blue 488, red 631)
Detectors:  7 ( forward & side scatter detectors, and 5 fluorescence detectors)
Colors: Up to 5 colors
Footprint: 24″ x 38.5″ x 35″
Weight: 187 lbs

FlowSight® – EMD Millipore (Amnis), best flow cytometers

FlowSight® – EMD Millipore (Amnis)

The FlowSight® Imaging Flow Cytometer stands apart from other flow cytometers by producing up to 12 images of each and every cell. The unique image collection system simultaneously produces darkfield (side scatter), brightfield, and up to 10 fluorescence images. The FlowSight® Imaging Flow Cytometer operates at ~20X magnification allowing visualization of fluorescence from the membrane, cytoplasm, or nucleus. Identifying cell conjugates or distinguishing single cells from doublets and debris is effortless.

Lasers: Up to 4 (488 blue, 405 violet, 561 green, 642  red)
Magnification: 20x 
Channels: 12
Footprint: 36” x 24” x 26”
Weight: 300 lb

 

Gallios™ – Beckman Coulter, best flow cytometers

Gallios™ – Beckman Coulter

The Gallios™ Flow Cytometer research system delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays.

Lasers: Available in 2, 3 or 4 laser configuration (standard blue 488 & red 638 with optional violet 405 & yellow 561)
Detectors:  7 ( side scatter, and 6 fluorescence detectors)
Colors: Available for 6-10 colors
Footprint: 23.8″ x 37.5″ x 27.6″
Weight: 230 lbs

Guava® easyCyte – EMD Millipore, best flow cytometers

Guava® easyCyte – EMD Millipore

The culmination of fifteen years of flow cytometry expertise, Guava® easyCyte instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers — and you choose the optical and sample-handling configurations that suit the needs of your lab. All instruments offer economical single-sample loaders, or a high-throughput multisample tray that acquires from a standard 96-well plate plus up to ten sample tubes.

Lasers: Available in 1, 2 or 3 laser configurations
Detectors:  Up to 14 parameters ( forward & side scatter, plus 12 fluorescence detectors)
Colors: Up to 12 colors
Footprint: 20″ x 10″ x 23.4″
Weight: 73 lbs

ID7000 – Sony Biotechnology, best flow cytometers

ID7000 – Sony Biotechnology

The Sony ID7000 is Sony’s newest and most capable spectral analyzer. It is configurable with up to seven different lasers (320, 355, 405, 488, 561, 637, and 808 nm) and 188 detectors. This instrument collects light from 360 nm to 920 nm and can support experiments of greater than 44 different colors, more than any other instrument available. In addition to Sony’s upgraded spectral unmixing software, a new feature offered is their spectral reference library feature which allows a user to store and use saved spectral data so that a user may run an experiment without needing to run reference samples. Additionally, this set up is configurable with an auto-sampler compatible with 96-well plates, 384-well plates, and a 24-tube rack with new agitation and cooling features.

Lasers: From three to seven lasers (320 deep ultraviolet, 355 ultraviolet, 405 violet, 488 blue, 561 yellow-green, 637 red, and 808 infrared)
Detectors: Up to 188
Colors: Over 40
Footprint: 41.7″ x 28.3″ x 30.3″
Weight: 485lbs

 

ImageStream® X Mark II – EMD Millipore (Amnis), best flow cytometers

ImageStream® X Mark II – EMD Millipore (Amnis)

The revolutionary ImageStream®X Mark II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This instrument produces multiple high-resolution images of every cell directly in flow, including brightfield and darkfield (SSC), and up to 10 fluorescent markers with sensitivity exceeding conventional flow cytometers.

Lasers: Up to 7 (488 blue standard, plus 375, 405, 561, 592, and 642 options)
Magnification: 20x or 60x options 
Channels: 12
Footprint: 35” x 26” x 25”
Weight: 400 lb

iQue®3 - Sartorius

iQue®3 - Sartorius

The iQue®3 is the newest Intellicyt (Sartorius AG) instrument with a focus on speed – during set up, acquisition, and analysis. The iQue®3 can sample a 96 well plate in under five minutes, and a 384 well plate in under 20 minutes at the bare minimum sampling requirements. It can aspirate as little as 1 µl of sample and it can record up to 35,000 events per second. This instrument comes in three laser configurations: Blue-Red, Violet-Blue-Red, or Violet-Blue-Yellow, and has up to 15 detection channels on the three laser systems. All configurations are all compatible with 96 and 384 well plates, while one configuration can accept 1536 well plates.

Lasers: Available in three configurations [488/640], [405/488/640], or [405/488/561]
Detectors: eight detection channels on two laser model or 15 detection channels on three laser models
Colors: Up to 13 on three laser model

iQue Screener PLUS – Sartorius

iQue Screener PLUS – Sartorius

The iQue Screener PLUS platform is an integrated instrument, software and reagent system that enables rapid, high content, multiplexed analysis of cells and beads in suspension. Fast comes standard with the iQue Screener PLUS. It excels for those screens where cells are precious or limited in number. For scientists who want to focus on revealing the biology, not on the technology used to get there, software-assisted automation and experiment-based analyses deliver the deep insight needed to answer complex biological questions.

Lasers: Available in 2 and 3 laser configurations (blue 488 & red 640 plus optional violet 405 or yellow 561)
Detectors:  8 (for 2 laser configuration) or 15 (for 3 laser configuration)
Colors: Up to 6 (for 2 laser configuration) or 13 (for 3 laser configuration)
Footprint: 39″ x 25″ x 26″
Weight: 180 lbs

 

LSR II – BD Biosciences

LSR II – BD Biosciences

The BD LSR II flow cytometer is fully configurable with one to four fixed-aligned, air-cooled lasers. By distributing a large number of fluorochromes over four lasers, you reduce spectral overlap and improve sensitivity. Fixed alignment provides consistent and stable day-to-day performance. The three new solid state lasers available on the BD LSR II flow cytometer—the Coherent® Sapphire™, Coherent VioFlame™ PLUS, and Lightwave Xcyte™— consume less power, produce less heat, and have a lower total cost over the life of the laser compared to gas-filled lasers.

Lasers: 4 (UV 355, violet 405, blue 488, red 633)
Detectors:  Up to 12 parameters
Colors: Up to 18

LSRFortessa™ – BD Biosciences

LSRFortessa™ – BD Biosciences

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.


Lasers: 
4 (UV 355, violet 405, blue 488, red 633) – Upgradable to 7 lasers
Detectors:  Up to 12 parameters
Colors: Up to 18
Footprint: 38″ x 36″ x 30″
Weight: 440 lbs

LSRFortessa™ X-20 – BD Biosciences

LSRFortessa™ X-20 – BD Biosciences

The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30″ x 29″ (76.2 x 73.7 cm) and a height of 30″ (76.2 cm).

The analyzer can be configured with up to 5 lasers to detect up to 20 parameters simultaneously to support ever increasing demands in multicolor flow cytometry. A wide range of up to 34 laser choices is available as excitation sources, including blue, red, violet, yellow-green, and UV

Lasers: Up to 5 (34 laser options)
Detectors:  Up to 20 parameters
Footprint: 30″ x 29″ x 30″
Weight: 440 lbs

 

MACSQuant® Analyzer 10 – Miltenyi Biotec

MACSQuant® Analyzer 10 – Miltenyi Biotec

The MACSQuant® Analyzer 10 was designed with automation in mind. It brings to life the versatility and power required for modern flow cytometry applications: an integrated magnetic column allows the enrichment of rare cells whereas several automation features enable truly hands-free operation, from automated start-up and cleaning to compensation and cell labeling.

Lasers: 405 nm (violet), 488 nm (blue), 635 nm (red)
Detectors:  FSC, SSC, 8 fluorescence channels
Colors: 8
Footprint: 23.8″ x 15.7″ x 15.4″
Weight: 110 lbs

MACSQuant® VYB Flow Cytometer – Miltenyi Biotec

MACSQuant® VYB Flow Cytometer – Miltenyi Biotec

On top of the performance, automation, compactness and convenience associated with the MACSQuant® Family of flow cytometers, the MACSQuant VYB comes with a uniquely configured optical bench, featuring violet, yellow, and blue lasers. It is a perfect match for labs utilizing fluorescent protein reporters or for labs that want to simply use PE and FITC conjugates.

Lasers: 405 nm (violet), 488 nm (blue), 561 nm (yellow-green)
Detectors:  FSC, SSC, 8 fluorescence channels
Colors: 8
Footprint: Width 605×400 mm (23.8×15.7″); Height 393–520 mm (15.4×20.4″)
Weight: 110 lbs

 

MACSQuant® X Flow Cytometer – Miltenyi Biotec

MACSQuant® X Flow Cytometer – Miltenyi Biotec

Engineered from the ground up as a compact flow cytometer, the MACSQuant® X was made to provide speed and reliability in high-throughput screening. 96-well plates are analyzed in under 15 min. , 384-well plates in less than one hour. Perfect for laboratories that want to maximize efficiency, while enjoying the certainty of getting reliable results from every run. Through the extended sampler, the MACSQuant X allows easy integration into plate hotels and liquid handling platforms. A true benefit in large-scale screening settings.

Lasers: 405 nm (violet), 488 nm (blue), 640 nm (red)
Detectors:  FSC, SSC, 8 fluorescence channels
Colors: 8
Footprint: Width 669×368 mm (26.3×14.5″); Height 393–582 mm (15.4×20.4″)
Weight: 110 lbs

 

MACSQuant® Analyzer 16 Flow Cytometer – Miltenyi Biotec

MACSQuant® Analyzer 16 Flow Cytometer – Miltenyi Biotec

The MACSQuant® Analyzer 16 is engineered to expand the revolutionary automation of the MACSQuant Analyzer line of flow cytometers. It facilitates fully automated data acquisition in a 24-tube or 96-well plate format. The instrument’s compact design is suited for basic research as well as advanced immune monitoring applications. It´s violet side scatter enhances small particle analysis improving analysis of exosomes and other extracellular vesicles.

Lasers: 405 nm (violet), 488 nm (blue), 640 nm (red)
Detectors:  FSC, SSC, 14 fluorescence channels
Colors: 14
Footprint: 605×400 mm (23.8×15.7″); Height 393–520 mm (15.4×20.4″)
Weight: 110 lbs

Navios – Beckman Coulter

Navios – Beckman Coulter

The Navios Flow Cytometer is a clinical system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. With superior detection capabilities, the Navios provides accurate measurements, especially for rare events, which is critical to clinical diagnosis and monitoring. The fully-featured, easy-to-use Navios software provides tools to improve the quality of results as well as optimize workflow efficiencies in a clinical lab.

Lasers: 3 (violet 405, blue 488, red 638)
Detectors:  9-12 (forward & side scatter, plus 7-10 fluorescent detectors)
Colors: Up to 10
Footprint: 38″ x 28″ x 24″
Weight: 230 lbs

 

Northern Lights - Cytek® Biosciences

Northern Lights - Cytek® Biosciences

The Cytek Northern Lights is Cytek’s newer base model that came after the Aurora. This model comes equipped with one laser and nine colors but can be upgraded to three lasers and 24 colors. This model is meant to be simple and budget friendly while remaining highly capable as backed by Cytek’s proprietary CWDM technology and software.

Lasers:  One (488 nm), two (488, 640), or three (405, 488, 640) laser configurations available
Channels: Up to 26 channels of detection over the full emission spectra (24 fluorescent plus FSC and SSC)
Colors: Up to 24 colors
Footprint: 21.3″ x 20.5″ x 20.5″
Weight: 134 lbs.

NovoCyte® – ACEA Biosciences

NovoCyte® – ACEA Biosciences

The ACEA NovoCyte® flow cytometer system is a high performance benchtop flow cytometer designed for all level of users and all types of laboratories. This budget-friendly instrument is capable of detecting up to 15 colors with impressive sensitivity and resolution. The customizable laser and optical configuration of NovoCyte offers high degree of flexibility while providing complex cell analysis capabilities. The NovoExpress software enables intuitive and easy sample acquisition and analysis. It allows simultaneous sample acquisition and data analysis. The NovoSampler Pro is an optional high throughput system that provides versatility in acquiring multiple sample formats such as single tubes, multiple tubes, 24-, 48-, 96-well plates in a single, simple setup.

Lasers: 1-3 (violet 405, blue 488, red 638)
Detectors:  15 parameters
Colors: Up to 13
Footprint: 23.6″ x 17.7″ x 15.4″
Weight: 86 lbs

S1000EON – Stratedigm

S1000EON – Stratedigm

Stratedigm’s customizable, upgradeable, and future-proof S1000EON (pronounced “S-1000-eon”) 4th generation flow cytometer is built to provide you with the ultimate in scalability and performance. The breakthrough result is an exceptional analyzer with patented optics, fluidics, and electronics configurable with up to 6 lasers and 30 parameters. Its industry-leading sensitivity enables the S1000EON to resolve dim events, making it the perfect choice for microparticle analysis and other demanding applications. And whether you purchased an S1000, S1000EX, or S1000EXi, Stratedigm has you covered; the S1000 platform can be upgraded to the latest Stratedigm technology any time after purchase.

 

Lasers: Up to 6—375 nm near-UV, 405 nm violet, 488 nm blue, 532 nm green, 552 nm olive, 561 nm yellow, 640 nm red, 805 nm IR
Parameters: Up to 30
Detectors: Quad-FSC and Dual-SSC plus 7 multiplex fluorescent detectors
Footprint: 21.5″ x 21″ x 24″
Weight: <74 lbs
Links: https://stratedigm.com/s1000eon-flow-cytometer/
 

SE520EXi – Stratedigm

SE520EXi – Stratedigm

Stratedigm’s SE520EXi series is a 2-laser, 6-color cytometer that gives uncompromised performance for flow users who run demanding, routine applications—without cutting corners. When you’re looking for the perfect fixed configuration flow cytometer, you should never have to sacrifice dependability and performance just to stay on budget.

Lasers: 2 – 488 nm blue laser, 640 nm red laser
Colors: 6: forward & side scatter plus 6 fluorescence channels
Detectors: FCS and SSC plus fluorescence detectors
Footprint: 21.5″ x 21″ x 24″
Weight: <70 lbs
Links: https://stratedigm.com/se520exi-flow-cytometer/

 

SA3800 – Sony Biotechnology

SA3800 – Sony Biotechnology

The Sony SA3800 spectral cell analyzer is Sony’s mid-level instrument. It supports up to four lasers while a 32 channel PMT array collects wavelengths of light from 420 nm to 800 nm. It incorporates advanced electronics, patented optical technologies and many points of automation to deliver true workflow simplicity. In addition to the hardware, Sony offers a unique software feature called “Global Standardization mode”. This allows users at different sites to standardize their instruments to a single baseline to eliminate instrument variability across instruments and sites.

Lasers: 1- 4 (violet 405, blue 488, yellow 561, red 638)
Detectors: FSC, SSC, FL 32chPMT (500 to 800nm), Violet x2ch PMT (420-500nm)
Footprint: 26″ x 26.5″ x 25″
Weight: 210 lbs

SP6800 – Sony Biotechnology

SP6800 – Sony Biotechnology

The Sony SP6800 is Sony’s original spectral model and was one of the pioneering spectral instruments on the market. The SP6800 is equipped with two to three lasers and a 32 channel PMT array that collects wavelengths of light from 420 nm to 800 nm on the three-laser model. This model is set up more simply than the others in the Sony lineup, but has proven to be very capable and has provided great resolution while accurately sorting out challenging color combinations.

Lasers: 2-3 (violet 405 and blue 488 with optional red 638)
Detectors: 66 data  point parameters (FSC, SSC, with prism array spectroscopy to 32 channel PMT)
Footprint: 23.6″ x 25″ x 28″
Weight: 70 lbs

Quanteon – ACEA Biosciences

Quanteon – ACEA Biosciences

The NovoCyte Quanteon™ benchtop flow cytometer is the ideal instrument for today’s high-end multi-color flow cytometry assays. Equipped with 25 fluorescence channels from 4 lasers, the Quanteon offers superior sensitivity, resolution, and automation-ready high-throughput capabilities with walk-away operation to simplify your workflow.

Lasers: 4 (violet 405, blue 488, yellow 561, and red 640)
Detectors:  25 independent silicon photomultiplier channels for up to 27 parameters
Footprint: 24.4” x 18.1” x 18.9”
Weight: 96 lbs

 

ZE5™ – Bio-Rad Laboratories

ZE5™ – Bio-Rad Laboratories

The ZE5 Cell Analyzer is an innovative new flow cytometer with flexible configurations to meet a broad range of experimental complexities and throughput needs. Whether it’s a low-complexity, 2-parameter experiment or a high-complexity, 28-parameter experiment, the ZE5 Cell Analyzer provides the industry’s first truly expandable flow cytometer. This flexibility makes the ZE5 Analyzer accessible for novice flow cytometry users yet flexible enough for the most experienced flow cytometry professionals.

Lasers: Up to 5 (UV 355, violet 405 and blue 488, yellow 561 and red 640)
Colors: Up to 30
Footprint: 29″ x 28″ x 27″
Weight: 240

Cell Sorters

Bigfoot - Propel Labs

Bigfoot - Propel Labs (Now part of Thermo Fisher Scientific)

The Propel Labs Bigfoot Spectral Cell Sorter is the most recent entry into the spectral market. The Bigfoot is highly configurable with up to nine lasers and 60 detectors and has many integrated features that make it well suited to high throughput applications. It has an integrated Class II biocontainment cabinet with wash station, on-board calibration beads, built-in agitation, and temperature control. The Bigfoot can go from 6-way sorting into tubes, to 4-way sorting into a 96-well plate, or 8-way sorting into a 384-well plate.

Lasers: Up to nine lasers (349, 405, 445, 488, 532, 561, 594, 640, 785)
Detectors:  Up to 20 detectors
Colors: Up to 60
Footprint: H 69.7″ x W 39″ x D 39″
Weight: 882 lbs

COPAS Flow Pilot™ - Union Biometrica

COPAS Flow Pilot™ - Union Biometrica

The COPAS FP platform is designed for the measurement of objects ranging in size from 20–1500 microns (diameter). Each of the four COPAS FP models features a fixed, specially engineered fluidic path, flow cell and optics optimized for a specific subset of that size range to provide maximum speed, accuracy, resolution and throughput, depending on the application. Instruments can dispense objects either into bulk receptacles or multiwell plates. A 488 nm solid state laser is used to measure both the size and optical density of the objects, as well as for fluorescence excitation. Each COPAS FP system can be equipped with up to three lasers. The real-time analysis of these measured parameters is used to make sort decisions and only those objects meeting the user-selected sort criteria are gently dispensed into multiwell plates or stationary receptacles. Those objects not meeting the sort criteria are diverted by a puff of air to a sample recovery container, where they may be recaptured, unharmed and still viable.

Available in 4 models, optimized for sample sizes:
FP-250 (250 µm)
FP-500 (500 µm)
FP-1000 (1000 µm)
FP-2000 (2000 µm)
Lasers:  Up to 3 (Blue 488, plus 2 optional – choose from 375, 405, 445, 561, 640 & 660)
Footprint: 29″ x 24″ x 24″
Weight: 100 lbs.

FACSAria™ III - BD Biosciences

FACSAria™ III - BD Biosciences

The BD FACSAria™ III sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. The fluidics and optical systems are precisely integrated to maximize signal detection. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve unrivaled sensitivity and resolution.

Lasers: Up to 6 (Blue 488 & Red 633 are standard, other lasers are optional)
Detectors:  Up to 20 detectors
Colors: Up to 18
Footprint: 28” x 28” x 48”
Weight: 400 lbs

FACSAria™ Fusion - BD Biosciences

FACSAria™ Fusion - BD Biosciences

The BD FACSAria™ Fusion flow cytometer delivers exceptional multicolor performance and ease-of-use. First introduced as the BD FACSAria™ sorter in 2003, this sorting knowhow is combined with best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. The fully integrated biosafety cabinet meets emerging operator and sample protection requirements as well as global standards for bioprotection.

Lasers: Up to 6 (Blue 488 & Red 633 are standard, other lasers are optional)
Detectors:  Up to 20 detectors
Colors: Up to 18
Footprint: 56” x 50” x 34”
Weight: 475 lbs
(without BioSafety Cabinet option)

FACSCalibur™ – BD Biosciences

FACSCalibur™ – BD Biosciences

The BD FACSCalibur™ platform allows users to perform both cell analysis and cell sorting in a single benchtop system. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells, and reticulocytes.

Lasers: 2 (Blue 488 & Red 635)
Detectors:  Up to 6 detectors (4 fluorescent)
Colors: 3 or 4
Footprint: 36” x 24” x 26.5”
Weight: 240 lbs

FACSVantage™ – BD Biosciences, best flow cytometers

FACSVantage™ – BD Biosciences

BD Biosciences designed the BD FACSVantage SE system with unique image collection optics that provide high sensitivity and resolution. Using up to three independent laser beam spots, you can collect as many as six fluorescence signals per cell. The system’s innovative pulse processing technology, available as an option, allows you to measure the area, width, and ratio of detector pulses. Pulse processing can be used to detect doublets in DNA analysis and produces the ratio of two fluorescence signals for use in calcium flux measurements

Lasers:  Up to 3
Colors: Up to 6
FACSJazz™ - BD Biosciences

FACSJazz™ - BD Biosciences

The BD FACSJazz™ is a cell sorter that features dependable BD performance, a benchtop fit, and simplified operation at an affordable price. Factory-optimized settings, intuitive alignment, real-time video monitoring, BD FACS™ Accudrop technology, and BD FACS™ Sortware sorter software provide comprehensive control during acquisition, sorting, and analysis.Together these capabilities make the most commonly used sorting applications accessible to researchers with limited flow cytometry experience.

Lasers: 2 or 3 (488 comes standard, 405, 561 & 640 are optional)
Colors: Up to 6
Footprint: 21” x 20” x 20”
Weight: 125 lbs

FACSMelody™ - BD Biosciences

FACSMelody™ - BD Biosciences

The BD FACSMelody™ cell sorter makes the complex world of flow cytometry and sorting accessible to more researchers, enabling deep scientific insights, lab efficiency and cost savings. Incorporating technology exclusive to BD, the BD FACSMelody cell sorter is based on the proven BD FACSAria™ platform of cell sorters. With fixed alignment and gel-coupled cuvettes, BD FACSAria sorters have a reputation for driving superior performance and reliability

Lasers: 2 or 3 (488 & 640 come standard, 405 is optional)
Detectors: Up to 11 (9 fluorescent)
Colors: Up to 9
Footprint: 19.5” x 22” x 19”
Weight: 90 lbs

FACSymphony S6 - BD Biosciences

FACSymphony S6 - BD Biosciences

The BD FACSymphony S6 is takes the best components from other BD instruments and combines them into a superior cell sorter. Cell sorting system components from the reliable FACSAria Fusion Cell Sorter were combined with optical components from the FACSymphony A5 Cell Analyzer to deliver reliability and a powerful optical system. This instrument can measure up to 60 parameters to define cells and sort them up to six ways or into plates or slides. This machine is configurable with up to nine spatially-separated lasers from over 25 different wavelength options and high parameter cascade (HPC) arrays allow for measurement of up to 20 parameters on a single laser line. Additionally, filters are removable and customizable.

Lasers: Up to nine spatially-separated lasers from over 25 wavelength options
Detectors: Measure up to 60 parameters while HPC arrays allow for up to 20 parameters on a single laser line

Influx™ - BD Biosciences

Influx™ - BD Biosciences

The flexible BD Influx™ cell sorter easily adapts to a researcher’s application or environmental requirements. A modular architecture, hands-on controls, support for up to ten lasers, exchangeable detector options, high throughput, and six-way sorting make the system highly configurable to precise needs of advanced research. In addition, BD FACS™ Sortware sorter software gives researchers comprehensive control, from configuration and compensation setup to acquisition, sorting, and analysis.

Lasers: 5 – 10
Detectors: Up to 24 parameters
Colors: 14 – 24 (depending on number of lasers)
Footprint: 72” x 62” x 48”
Weight: 229 lbs

JSAN™ – Bay Bioscience

JSAN™ – Bay Bioscience

The JSAN JrSwift™ is a complete cell sorting system known for its affordability, flexibility, and ease of use. It has a simple optical alignment capable of housing up to two lasers and detecting six colors while sorting at high speeds – detecting 20,000 events per second at 30 psi. This is a complete system; included with the instrument are all the necessary components: the compressor, vacuum pump, computer, high resolution display, and software.

Lasers: 488 Blue laser standard, optional choice of second laser (UV, Violet, Yellow, Red)
Detectors: Up to eight (two light scatter, six fluorescence)
Colors: Up to six
Footprint: 27″ x 28″ x 25″
Weight: 265 lbs

JSAN JrSwift™ – Bay Bioscience

JSAN JrSwift™ – Bay Bioscience

The JSAN JrSwift™ is a complete cell sorting system known for its affordability, flexibility, and ease of use. It has a simple optical alignment capable of housing up to two lasers and detecting six colors while sorting at high speeds – detecting 20,000 events per second at 30 psi. This is a complete system; included with the instrument are all the necessary components: the compressor, vacuum pump, computer, high resolution display, and software.

Lasers: 488 Blue laser standard, optional choice of second laser (UV, Violet, Yellow, Red)
Detectors: Up to eight (two light scatter, six fluorescence)
Colors: Up to six
Footprint: 27″ x 28″ x 25″
Weight: 265 lbs

MA900 Multi-Application Cell Sorter - Sony Biotechnology

MA900 Multi-Application Cell Sorter - Sony Biotechnology

The Sony MA900 Cell Sorter is a simple and versatile instrument sufficient for most sorting applications. Analyze up to 14 parameters from four lasers and sort into a wide variety of tube and plate setups with a high degree of built-in automation.

Lasers: Four lasers (488 blue, 638 red, 405 violet, and 561 green-yellow)
Detectors: 14 (12 fluorescent and two scatter)
Colors:
Footprint: 21.7″ x 21.7″ x 28.4″
Weight: 231 lbs

MACSQuant® Tyto® Cell Sorter – Miltenyi Biotec

MACSQuant® Tyto® Cell Sorter – Miltenyi Biotec

The MACSQuant® Tyto® is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for high speed, 10-parameter cell sorting. A unique feature of the instrument is the fact that the actual sorting process takes place exclusively within a single-use, disposable and fully closed system: the MACSQuant Tyto Cartridge. Sorting on the MACSQuant® Tyto® is aerosol and droplet-
free. The closed cartridge provides full operator safety and eliminates the risk of sample contamination and carry-over. In addition, all consumables are available in GMP format including GMP regulatory documentation (CoA, TSE, CoO and PIF) allowing an easy transition from a research to a clinical setting.

Lasers: 405 nm (violet), 488 nm (blue), 638 nm (red)
Detectors:  BSC, SSC, 8 fluorescence channels
Colors: 8
Footprint: (WxDxH) 101 x 56 x 26 cm
Weight: 110 lbs
Flow rate: up to 110,000 cells/s

MoFlo® Astrios™ EQ - Beckman Coulter

MoFlo® Astrios™ EQ - Beckman Coulter

The MoFlo® Astrios EQ was designed around the needs of today’s research scientists — broader analytical capability, robust biohazard containment, application flexibility, and consistently trustworthy data. The MoFlo Astrios EQ is easy, intuitive, and flexible — and lets you see what other sorters cannot. With the MoFlo Astrios EQ, you never have to be limited by your instrumentation. Its patent-pending dual enhanced forward scatter (eFSC) technology enables you to see and sort particles you might otherwise miss. Measure crucial particle characteristics over more than three orders of magnitude, from as small as 200 nm to 30 µm diameter, all at the same time. You can also trust MoFlo’s proven fluidics to be gentle with your biological material.

Lasers: 7 lasers, 2 alignment stages (355, 405, 488, 532, 561, 592, 642)
Detectors: Up to 44 parameters simultaneously (out of 51 total options)
Capable of 6-way sorting

MoFlo® XDP - Beckman Coulter

MoFlo® XDP - Beckman Coulter

Compatible with an array of laser options, MoFloTM XDP delivers unmatched sorting capability with high viability, yield and purity at 100,000 analysis rate events/second. The open architecture of the MoFlo XDP accommodates diverse sorting applications to meet the most demanding research need. The MoFlo XDP can accommodate most visible wavelength laser lines in addition to UV and IR and with an enhanced optical design, it delivers sufficient sensitivity and resolution to reimagine multi-parametric cell sorting.

Lasers: 3-5 (355, 405, 488, 532 & 640)
Detectors: Up to 17 (14 fluorescent)
Colors: Up to 15
Capable of 2 and 4 way sorting

S3e™ - Bio-Rad Laboratories

S3e™ - Bio-Rad Laboratories

The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts. This compact system is equipped with either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.

Lasers: 1 or 2 (488 standard, plus 561 or 640)
Detectors: Up to 6 (4 fluorescent)
Colors: Up to 4
Footprint: 27.5″ x 25.5″ x 25.5″

SH800S - Sony Biotechnology

SH800S - Sony Biotechnology

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes and applications using the 70 μm, 100 μm, and 130 μm microfluidics sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use. System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third-party analysis tools.

Lasers: 1 or 2 (488, 405, 638 and 561)
Detectors: Up to 8 (6 fluorescent) parameters
Footprint: 21.5″ x 21.5″ x 28.5″
Weight: 216 lbs

SY3200 – Sony Biotechnology (iCyt)

SY3200 – Sony Biotechnology (iCyt)

The iCyt SY3200 system is one of the most innovative and versatile Flow Cytometry Research platforms available in the market. The innovative and flexible laser delivery array allows for ultimate laser compatibility, while the unique reflective collection optics ensure maximum fluorescence collection efficiency. Powerful, Sony designed electronics enable collection of high resolution data and ultra high speed cell sorting while maintaining superior sensitivity. Plug and Play PMTs in the Scalable PMT Array module provide the ultimate flexibility in collection options while minimizing cost. The SY3200 provides true innovation to deliver a comprehensive cell sorting solution that meets the challenges of the increasing complexity of research flow cytometry applications.

Lasers: 4-6 (violet 405 and blue 488, yellow 561 and red 640)
Colors: Up to 30

WOLF Cell Sorter – NanoCellect Biomedical

WOLF Cell Sorter – NanoCellect Biomedical

The WOLF Cell Sorter has a basic optical set up and comes in a very small size, but it has many unique features that make it a compelling instrument. The instrument comes with one 488 nm blue laser, three fluorescent detectors, and two scatter detectors. As for the unique features, the WOLF operates at under two psi which is 30 times gentler than any conventional cell sorter or cytometer which greatly improves cell viability. The WOLF also safeguards against contamination by employing disposable microfluidics cartridges and a novel microchip sorting system that is also disposable.

Lasers: One 488 Blue laser
Detectors: 3 fluorescence, 2 light scatter
Colors: Up to 3
Footprint: 14.3″ x 15.3″ x 15.1″

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