Flow cytometry remains the most powerful tool for studying apoptosis in cell populations. However, many researchers may not be aware of the variety of options available for apoptosis markers. Here we explore the use of flow cytometry in apoptosis research and examine...
Cytometer technology is continually improving, with more lasers, detectors, and channels to allow the measurement of more parameters. But researchers are too often reluctant to expand the scope of their experiments for fear of increasing design complexity. This month...
This month we explain why antigen density is important to flow cytometry studies, describe how it is measured, and list some useful references for known antigen expression densities… 1. Why Antigen Density Matters 2. Methods for Determining Antigen Density 3....
Getting started in flow cytometry may seem overwhelming, so we’ve broken the flow cytometry experiment down into seven basic steps. 1. Select your Cytometer 2. Design your panel 3. Optimizing your staining protocol 4....
The field of flow cytometry research has evolved significantly over the past 70 years. Here we take a look back at some of the most important achievements on the path to bigger, faster and better flow cytometry experiments. Microscopes (Early 1700s) Antonie van...
The commercial availability of antibody-linked fluorescent dyes has expanded rapidly over the past decade. Where researchers were once limited to a handful of common dyes (FITC, PE, APC, PerCP, etc.), they can now choose from an extensive list of fluorescent dye lines...
