Reagents for Super-Resolution Microscopy

Reagents for Super-Resolution Microscopy

Written by Sarah Locknar Reagents for Super-resolution microscopy Super-resolution microscopy (SRM) methods have taken the microscopy world by storm (literally!). These techniques enable imaging an order of magnitude beyond the optical “Raleigh limit”. There are two...
Invisible Imaging: Fluorescence Microscopy in the UV and NIR

Invisible Imaging: Fluorescence Microscopy in the UV and NIR

Written by Sarah Locknar The whole-body fluorescence imaging of a mouse IV-injected with the NIR-II dye IDSe-IC2F encapsulated via PEGylation, excited with 793 nm laser light. Used under CC by 4 license without modification from (Feng, 2021) Figure 8k Invisible...
Clearing Tissue for Microscopy: Part 2 – Hydrophilic Methods

Clearing Tissue for Microscopy: Part 2 – Hydrophilic Methods

Cover Image: E12.5 whole mouse embryo was immunostained with neurofilament antibody and cleared with RTF. Scale bar, 1000 μm. Used without modification under Creative Commons Attribution 4.0 International License from (Yu T. Z., 2018) Figure 4a. As explained in last...
Clearing Tissue for Microscopy: Part 1 – Dehydration Protocols

Clearing Tissue for Microscopy: Part 1 – Dehydration Protocols

Cover Image: Imaging of the whole-brain vasculatures at sub-cellular resolution. Brains of adult Tie2-Cre;tTAflox;tetO-H2BGFP (TTH) mice were cleared using the PEGASOS method and imaged with a tiling light-sheet microscope. g An X-Z optical slice acquired at the...